Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	647,057	G→A	G151G (GGC→GGT)	citG ←	triphosphoribosyl‑dephospho‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	647,057	0	G	A	100.0%	25.0 / NA	10	G151G (GGC→GGT)	citG	triphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/3);  total (7/3)

GCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATG  >  NC_000913/646817‑647165                                                                                                                                                                                                                                                 |                                                                                                             gcgcgATGCAACGTTGGAATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCAAGTTCAGGATCTACCCCCTGATCCAGCAGAGTGAGGTAATGCGACAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGACCACGGc                                                                                                       >  1:138857/1‑248 (MQ=255)        ncAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTACGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGACCACGGCAGAAACTTgc                                                                                             >  1:32032/2‑251 (MQ=255)                gggaTCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTGCAGGACCTAACCCCTGATCCAGAAGAGTGAGGTAATGCGGCAAGGCGTGATTGAGCACCAGTGGATACCCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGTTGTTGGTACAACCTTTGCCCTGCCGCCAGTGGTAAATTATGGTTACGCAGTTCGCGATCGGTCAGACCACGGCAGAAACTTGCCGCCGTAg                                                                                     <  1:162896/248‑1 (MQ=255)                 gTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAACCCTGCTCACGAAGAGTGAGCTAATGCGGTAAAGCGTGATTGATCACCAGTGGATAAACCCCTTCGGCTTCACCGCGAGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCCGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGACCACGGCAGAAACTTGCCGCCGt                                                                                       >  1:23698/1‑248 (MQ=255)                      gCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGAAGAGTGAGGTAATGCGGAAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCCCCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGACCACGGCAGAAACTTGCCGCCGTAGaa                                                                                   >  1:61993/1‑247 (MQ=255)                                                                                                                                        aCCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCTTCAGTTGTGAATTATTGGTACGCTGTTCGCGATCGGTCAGACCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGgttgagttg                                                <  1:67767/168‑1 (MQ=255)                                                                                                                                                             tGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGACCACGGCAGAAACTTgc                                                                                             >  1:3240/1‑102 (MQ=255)                                                                                                                                                                   ngTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGACCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAAtg  >  1:41619/2‑187 (MQ=255)                                                                                                                                                                                                                                            gTCAGACCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGgttggttg                                                     <  1:5547/63‑1 (MQ=255)                                                                                                                                                                                                                                            gTCAGACCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGgttggttg                                                     >  2:5547/1‑63 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                             GCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACCAGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTTGCCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATG  >  NC_000913/646817‑647165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑