Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,970	A→G	T186A (ACA→GCA)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,970	0	A	G	88.9%	26.0 / NA	9	T186A (ACA→GCA)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (6/2);  minor base T (0/1);  total (6/3)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTAC  >  NC_000913/3268750‑3269211                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  gTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAgn                                                                                                                                                                                                                     <  1:99775/251‑2 (MQ=255)    aaaaaaCCAGGAAATATCCAAAGGTAATATCAATATTAATTGCACATTAAAAGATAATTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATCTAGATTATTATGATTTCACAGATAAACATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGtt                                                                                                                                                                                                                   >  1:164963/1‑250 (MQ=255)                                              cccATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTACTTGTGCCACTGGAGCTATCGTTGATCTCTCTTCTATGGCCTTAAGTACTGTCGATTTCGATTCTTATGATTTCACAGATAACCATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCTACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGcc                                                                                                                                                                                        >  1:84608/1‑235 (MQ=255)                                                cATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATACTATCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGattat                                                                                                                                                                      >  1:148003/1‑251 (MQ=255)                                                                    naaaTCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGt                                                                                                                                                  >  1:18418/2‑251 (MQ=255)                                                                                ttAAACGTAATTTTTAGTTGTGGGACTGGAGATAGCGTTGATCAAGCCTCTCTGGACTTACGTAATGTCGAGTTAGATTATTAGGATTTCACAGATAAACAGAGGGCTA‑TATCTATTTAAAATCCTTATAAATGGAATTCATCAAATCTTGTTAATGCCAACATGTGCCAGGTGAATTTTGTTAGTACAACACAAAACGCCACAATCTCCTGGGa                                                                                                                                                                          <  1:89515/215‑1 (MQ=255)                                                                                                        actgtataaatCGTTGATCTATCCTCTCTGGACTTACTTAATGGCGATTGAGATTATTATGATGTCACAGATAAACATATGGCTAATA‑CTATTTTAAAGCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTGCAGGTTAACTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGat                                                                                                                                                                         <  1:106508/183‑1 (MQ=255)                                                                                                                                                                                        ggCTAATA‑CTATTTTACATCCTTTTAAATTGAATTCAGCAAATTTTACTAATGCCAACATGTTTCAGGATAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAAtt                               >  1:36685/1‑250 (MQ=255)                                                                                                                                                                                                                     ttGAAATCAGCAAATTTTACTAATGCCAACATGTTTCAGGATAATTTTGTTAGTTCAACACAAAACGCAACAAACTCCTGAGATTATTTACTAAAAATAACGCCTGTTTTAATCAGCCTTAGCGATATGCATTATGAAGAACAAATCATGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAACAATTAAACATTATGAGATTTGCAATTATAAAATCTATACCAAGGGAAAATCTTAc  >  1:167471/1‑251 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  GTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATA‑CTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAACAATTAAAAATTATGAGATTTGCAATTATAAAATCTATACCAAGGGCAACTCTTAC  >  NC_000913/3268750‑3269211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑