Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,532	G→A	S311N (AGT→AAT)	wzyE →	enterobacterial common antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,532	0	G	A	100.0%	21.6 / NA	9	S311N (AGT→AAT)	wzyE	enterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

GGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGG  >  NC_000913/3979287‑3979712                                                                                                                                                                                                                                                      |                                                                                                                                                                                     ggcggcggcGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATgg                                                                                                                                                                                 <  1:148791/251‑1 (MQ=255)                                                      ncTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGCCACCTTCTCGCCGTGGGAGCATCTGGCGTTGCTGTTGCAGAACTACGACACCATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGCTTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCgg                                                                                                                             >  1:16987/2‑250 (MQ=255)                                                                                          ataCGTTTCTCTATCTCACTCGCGACGCCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACGCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACtg                                                                                           <  1:162814/248‑1 (MQ=255)                                                                                            aCGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGTACTACGACAACATCGACGTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGTAAGTGCTGAATAACCACTCCGGACTGGCTATCTCGCCTACGCTTATAGTCTCACtggtgg                                                                                       <  1:140541/250‑1 (MQ=255)                                                                                                                                                          gAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGACGGCGCGTTGTTCATCCCGCTCGGGGCGATCGtgg                                             >  1:145772/1‑230 (MQ=255)                                                                                                                                                                cGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGACGGCGCGTTGTTCATCCCGCTCGGGGCGATCGtggtt                                           >  1:51714/1‑226 (MQ=255)                                                                                                                                                                               ccAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGACGGCGCGTTTTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTg    <  1:156446/250‑1 (MQ=255)                                                                                                                                                                                                       cgATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGACGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTggg  >  1:129489/1‑228 (MQ=255)                                                                                                                                                                                                                              ccTGGCTGTGGCCGGGTCGCCCGAATATGGTGCTGAACTCCGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTAc                                                                                                           >  1:91371/1‑100 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                     GGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGG  >  NC_000913/3979287‑3979712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑