Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	326,658	C→T	R197H (CGC→CAC)	betA ←	choline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	326,658	0	C	T	90.0%	16.3 / ‑5.4	11	R197H (CGC→CAC)	betA	choline dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (4/5);  minor base G (0/1);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCCAGCAGTTCAGCGTTGCCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTTTGGAGGTAGTGACGCTCACCGGGCCATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCG  >  NC_000913/326412‑326864                                                                                                                                                                                                                                                       |                                                                                                                                                                                                               gCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACg                                                                                                                                                                                                            <  1:64096/251‑1 (MQ=255)                                   gCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGATCCAGCGGAcc                                                                                                                                                                           <  1:45578/250‑1 (MQ=255)                                          cTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCCTTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGTTCCATCGGACCAAAACCtt                                                                                                                                                                   >  1:148835/1‑251 (MQ=255)                                                     naaTCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCAGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATa                                                                                                                                                         >  1:43015/2‑250 (MQ=255)                                                         cgcgCCTGCACATAACAGCCCTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCAGTCGACGCCCACCGCGCGTTTGCCGTCAAAAAGGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTGAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGt                                                                                                                                                    <  1:24740/251‑1 (MQ=255)                                                             ccTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGAGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGTTg                                                                                                                                                  >  1:155211/1‑249 (MQ=255)                                                             ccTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGAGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAACAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGTTg                                                                                                                                                  >  1:65021/1‑249 (MQ=255)                                                                                  gctcgtccgatcgcgcGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGAACAAAACCTTCCTGCGGATAACCGTTGAGATCGTCCGTGCGCGGGTAGcc                                                                                                                           <  1:36547/239‑1 (MQ=255)                                                                                                                                      cGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCCCTCATCGCTTGAGTCCGCATGGTCAGGTTAGGCCGCGATTTGGCCTAATCGAGATAGCCACGCGCGGTGCTGGCGTGAAGGCACTACGGCGTAACGGTGCGCTCCATCGGGCCACAACCTTCCTGCTGATAACCGTTGCGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCACACAGCGGATTGACACCgggg                                                                       >  1:77430/1‑250 (MQ=255)                                                                                                                                                                            gTCATAGCGTGAGTACGAAGGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGTTGAGATCGTCCGGGCGCGGGTAGCCCGCCTGc                                                                                                                    <  1:50992/168‑1 (MQ=255)                                                                                                                                                                                                           gccgcgTTTTGGCCTGATCGGGATAGCCCCGCGCGGTGTTGGCGGGCCGGCCCTGCGGCGGGCCGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGTTGAGATCTTCCGGGCGCGGTGAGCCCGCCTGCACGCCCGGTTCAATCATCGCTTCAAACAGCGGATTGACGCGGGGTTTGGAGGGAGGGACGCGCCCCGGGCCATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCg  <  1:80040/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                               GCCAGCAGTTCAGCGTTGCCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGGACCAAAACCTTCCTGCTGATAACCGTTGAGATCGTCCGTGCGCGGGTAGCCCGCCTGCACGCCCGCTTCAATCATCGCTTCAAACAGCGGATTGACGCCGGGTTTGGAGGTAGTGACGCTCACCGGGCCATCACCGCCGTGATAGTCGTTTTCACCCATATCGCGAGTCTCG  >  NC_000913/326412‑326864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑