Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,783,814	G→A	D280N (GAT→AAT)	fadK →	short chain acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,783,814	0	G	A	100.0%	19.4 / NA	8	D280N (GAT→AAT)	fadK	short chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

CGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAGGGTCTGCCAAAGGGCGTGATGCTAACGCATAACAATATTCTCGCCAGTGAGCGGGCTTATTGCGCGCGACTGAATCTGACCTGGCAGGATGTCTTTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCTTTTGAATGTACTAGAGAAACAACCCGCGGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGT  >  NC_000913/1783572‑1784026                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                     cGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAGGGTCTGCCAAAGGGCGTGATGCTAACGCATAACAATATTCTCGCCAGTGAGCGGGCTTATTGCGCGCGACTGAATCTGACCTGGCAGGATGTCGTTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATTTTc                                                                                                                                                                                                              <  1:163991/251‑1 (MQ=255)                                                  cGGAACCGAGGGTCTGCCAAAGGGCGTGATGCTAACGCATAACAATATTCTCGCCAGTGAGCGGGCTTATTGCGCGCGACTGAATCTGACCTGGCAGGATGTCTTTATGATGCCTGCGCCAATTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTTAATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGt                                                                                                                                                              >  1:172389/1‑250 (MQ=255)                                                   ngAACCGAGGGTCTGCCAAAGGGCGTGATGCTAACGCATAAAAATATTCTCGCCAGTAAGCGGGCTTCTTGCGCGCGACTGAATCTGACCTGGCAGGATGTCTTTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTAt                                                                                                                                                            >  1:17341/2‑251 (MQ=255)                                                            ggTCTGCCAAAGGGCGGGATGCTAACGCATAACAATATTCTCGCCAGTGAGCGGGCTTATTGCGCGCGACTGAAGCTGACCTGGCAGGATGTCTGTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCAGTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATGTTCTCTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGgcgc                                                                                                                                                   <  1:159345/251‑1 (MQ=255)                                                                                                      gCCAGTGAGCGGGCTTATTGCGCGCGACTGAATCTGACCTGGCAGGATGTCTTTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCTTTTGAATGTACTAGAGAaac                                                                                                          >  1:81101/1‑250 (MQ=255)                                                                                                                                                  ggATGTCTTTATGATGCCTTCGCCCCTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATGTTCCCTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCACGGCGCAACGCCGTTTGGCTATGATCTTTTGAATGTACTAGAGAAACAACCCGCGGACCTTTCAGCGCTGCGTGTCTTTCTTTGCGGCGGaa                                                             <  1:55561/251‑1 (MQ=255)                                                                                                                                                        cTTTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCTTTTGAATGTACTAGAGAAACAACCCGCGGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACcaca                                                        >  1:72373/1‑250 (MQ=255)                                                                                                                                                                                                             cGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAAATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCTTTTGCATGTACTAGAGAAACATCCCGCGGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTCAATTATTAAgt  >  1:172721/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                     CGGCGATAACGACCCACGGCGATGAATTAGCTGCGGTGCTGTTTACCTCCGGAACCGAGGGTCTGCCAAAGGGCGTGATGCTAACGCATAACAATATTCTCGCCAGTGAGCGGGCTTATTGCGCGCGACTGAATCTGACCTGGCAGGATGTCTTTATGATGCCTGCGCCACTTGGTCACGCAACGGGCTTTCTGCATGGCGTAACGGCACCATTCTTAATTGGCGCTCGCAGCGTGTTGTTAGATATTTTCACTCCTGATGCGTGTCTCGCGCTGCTTGAGCAGCAGCGTTGCACCTGTATGCTCGGCGCAACGCCGTTTGTCTATGATCTTTTGAATGTACTAGAGAAACAACCCGCGGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGT  >  NC_000913/1783572‑1784026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑