Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,843,551	C→T	G80E (GGG→GAG)	adeQ ←	adenine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,843,551	0	C	T	100.0%	31.5 / NA	12	G80E (GGG→GAG)	adeQ	adenine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/1);  total (11/1)

GCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTT  >  NC_000913/3843317‑3843793                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGcc                                                                                                                                                                                                                                    >  1:138299/1‑251 (MQ=255)                                        aaaCTTAAGGGAATGTGGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACtt                                                                                                                                                                                              <  1:138475/250‑1 (MQ=255)                                               aGGGAATGCTGGAGATCATCCCGTACCGGATACGAAACAGCGTGAGCAAAAATAGTACAACTGCGCCCCCGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCAACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTcc                                                                                                                                                                                      >  1:139155/1‑251 (MQ=255)                                                                                                     gTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGACTGCCAGGAGATGAACATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGCGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCCTTTGTGc                                                                                                                                                                              >  1:116802/1‑205 (MQ=255)                                                                                                                  ncAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTc                                                                                                                    >  1:34027/2‑250 (MQ=255)                                                                                                                               cccATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACAACGAAGGCACAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCCCGGGTAAGTTAGCAAATCTCTCCATCGCAATACTGCCGATCCCGGCAATCAAACAGGTGGCAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGcaatca                                                                                                       >  1:171783/1‑250 (MQ=255)                                                                                                                                             ccAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCAATTGCCGGAGACAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCAGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATAAATTCTGTGCGGACgg                                                                                        >  1:121504/1‑251 (MQ=255)                                                                                                                                                                cccACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTAACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGt                                                                       >  1:120170/1‑249 (MQ=255)                                                                                                                                                                         acgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAaata                                                             >  1:34764/1‑250 (MQ=255)                                                                                                                                                                                                             gCCAGCGCCACGGGTAAGTTAGCAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGACATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTCAATAATCGCGAAAGCg                                                 >  1:9144/1‑226 (MQ=255)                                                                                                                                                                                                                                   cAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTACCAAACACCAATAAAGAGTCAATTTGTGCAGCGCCGAGGATTTGCGGGTTCACAAAAACGCTGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATc                                                          >  1:116533/1‑195 (MQ=255)                                                                                                                                                                                                                                   cAAATATCTCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCAttttt  >  1:137797/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GCGATAAATAATCCAATTCCGCTGGTGATACCAATACGTAAACTTAAGGGAATGTTGGAGATCATCCAGTACCGGATACGAAACAGCGTGAGCAAAAATAGTCCAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGAGGTAGTTTAAATAATCGCGAAAGCGTCCCTGATTCATTACTCACGTAATCGGTATTGTCATTATTCATTTTT  >  NC_000913/3843317‑3843793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑