Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,134	G→A	G250S (GGT→AGT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,134	0	G	A	100.0%	14.0 / NA	7	G250S (GGT→AGT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

GCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTG  >  NC_000913/70957‑71295                                                                                                                                                                                  |                                                                                                                                                                  gCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgag                                                                                          <  1:36636/251‑1 (MQ=255)     aCAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTGTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGTGCCAGCCcgagcgag                                                                                      <  1:58574/251‑1 (MQ=255)                                                                             gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGAGAAGCTGCTTTTGAGCACTACCCAGATGCATATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCAATTTCTAGCGCGTAATTAAAAAATGCACCGGGGCACGCCCGAGCGCGTTTCGTGCCGGTTGTGAAGAAAAAGTGCATGATGTAGCCGACAAGTTGTCATAATTGGTAACGCATCatac               >  1:62131/1‑247 (MQ=255)                                                                                   agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTTGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGAc        <  1:71780/251‑1 (MQ=255)                                                                                         ggATTAGCGTCTTAAGCTGGCGCGAGGAACAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTg  >  1:52918/1‑251 (MQ=255)                                                                                                                                                  tGAGCACTNCNNNGNNGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACNCTATTTCTCGCGAGTNTTNAAAANATGNACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAATAAGTGAATGATGTAGCCGTCAAGTTGTc                                    >  2:116668/1‑160 (MQ=255)                                                                                                                                                  tGAGCACTACCCGGATGCCTAGCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGATCGAGTTTCGTGCCGGTTGTGAAGAATAAGTGAATGATGTAGCCGTCAAGTTGTc                                    <  1:116668/160‑1 (MQ=255)                                                                                                                                                                                  |                                                                                                                                                                  GCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTG  >  NC_000913/70957‑71295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑