Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	643,399	G→A	I7I (ATC→ATT)	yldA ←	protein YldA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	643,399	0	G	A	100.0%	11.7 / NA	6	I7I (ATC→ATT)	yldA	protein YldA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

GTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGCCGGGGGGAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGT  >  NC_000913/643176‑643567                                                                                                                                                                                                                                |                                                                                                                                                                         gTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTACACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGCCGGGGGGAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGAATGTAAAATGCTTCTGCCATGGTTAtt                                                                                                                                               >  1:95429/1‑251 (MQ=255)                                                  gagTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTGCAGGTCTGGTGAATGCGATGCCGGGGGGAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGAATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAAc                                                                                              <  1:52017/251‑1 (MQ=255)                                                                              gTGCACAAAGCGCAGAGGCGGCGCAGTAGGCGGTTTCAGGTATGGTGAATGCGATGCCGGGGGGAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGAATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGACCCAGGa                                                                                         >  1:150789/1‑228 (MQ=255)                                                                                                                                gCGATGCCGGGGGGAACAATAGGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGAATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGAGTGTGCTGAAAGAGGATGCGAGTTCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATg                <  1:64121/251‑1 (MQ=255)                                                                                                                                           gggAACAATATGTTCGTCGGTAGGGGTTTTCGAGGTAAAGGACAGCCTTGTCGAGAATCGCCGCCATAATCAGAAATCCTATCAGAATGTAAAATGCTTCTGCCATGGTTATTcc                                                                                                                                             <  1:24679/115‑1 (MQ=255)                                                                                                                                              aaCAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGAATGTAAAATGCTTCTGCCATTGTTATTCCCACAAACGAAACGGAATAAGTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGt  <  1:110376/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                         GTTTGAAGAGGCCGCCCGGGGATATGAGATTTTCGAAAAACGTGAAGAGGAGTGCCGTAAGGTGATTCTGGTACCCGGTGCACAAAGCGCAGAGGCGGCGCAGAAGGCGGTTTCAGGTCTGGTGAATGCGATGCCGGGGGGAACAATATGATCGTCAGGAGTGGTTTTCGAGGTAAAGGACAGCCATGACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGT  >  NC_000913/643176‑643567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑