Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,994	G→A	G347D (GGC→GAC)	yeaH →	DUF444 domain‑containing protein YeaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,994	0	G	A	100.0%	37.3 / NA	15	G347D (GGC→GAC)	yeaH	DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/8);  total (7/8)

ATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCA  >  NC_000913/1869784‑1870223                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      aTCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGActc                                                                                                                                                                                                                  <  1:35929/232‑1 (MQ=255)    ngTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTTCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTg                                                                                                                                                                                            >  1:102716/2‑251 (MQ=255)                             ngTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTc                                                                                                                                                                   >  1:135852/2‑251 (MQ=255)                                             aTCCGNNATNNNACNNAGGCGAAAGAAGTCGATGAACATNAGTTTTTCTACTCGCAGGAAACAGGCGGCNCNANTGTTNNCAGNGCCCTGAAACNGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGACTATNTNCGCTGCACAAGCATCGGACGACGATAACTGGNCCGATGAc                                                                                                                                                                                                                    >  2:122236/1‑186 (MQ=255)                                             aTCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTTATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGCGGAATATTTACGCTGCTCAAGCATCGGACGACGATAACTGGGCCGATGAc                                                                                                                                                                                                                    <  1:122236/186‑1 (MQ=255)                                                           ccAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTAGCAGGAAACAGGCGGCACCATTGTTTCCAGAGCCCTGACACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGAGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCagtc                                                                                                                                     >  1:126232/1‑251 (MQ=255)                                                               gCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCGGGCGAAAAAAGTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGc                                                                                                                                 <  1:155421/251‑1 (MQ=255)                                                                                                    aGGAAACAGGCGACACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCa                                                                                             >  1:93628/1‑250 (MQ=255)                                                                                                                              aGCGCCCTGAAACTGATGGATTAGGTAGTGAAATAGCGTTATAACCCGGCACATTGGAATATGTACTCTGGACAAGCATCGGACGACGAGAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATcc                                                                                                                                                                                              <  1:112272/127‑1 (MQ=255)                                                                                                                                                           gAAAGCGCGTTATAACCCGGCACAGTNGAATATNNACNCTGCACAAGCATCGGACGANGANNNCNNNGNCGATGNCTNNCCGCTTTGCNATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCg                                                                                                                                                                  >  2:38464/1‑126 (MQ=255)                                                                                                                                                           gAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCg                                                                                                                                                                  <  1:38464/126‑1 (MQ=255)                                                                                                                                                            aaaGAGCGTTATAACCCGGTACAGTGGAATATTTACTCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCTCCGCTTTGCCATTAAATCCTGGCGGAAAAATTATTACCTGTAGTTCGTCATTACAGCTATATCGAAATTTCCCGTCGTGCACATCAGGCTTTGTGGCAACAATATGAGCATCGTCCATCTACTATCGACAACGTTACGATGCAGCACATCCGCGACCAGGATGATATGTATcc                                    <  1:167845/251‑1 (MQ=255)                                                                                                                                                                          cccGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGACGATAACTGGGCCGATGACTCGCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGATAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAAc                      <  1:50757/251‑1 (MQ=255)                                                                                                                                                                                   gTGGAATATTTACGCTGCACAAGCATCGGACGACGATACCTGGGCCGATGACTCTCCGCtt                                                                                                                                                                                                           >  1:155581/1‑61 (MQ=255)                                                                                                                                                                                              aCGCTGCACAAGCATCGTACGACGATAACTGGTCCTATGACTCTCCGCTTGGCCATGAAATCCTGGCGAAAATATTATTTCCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCCCAACAGACATTGTTGCGACAATATGGGCTTCTGTAATCTACTTTCGACATGATGGCGATGCAGCAGTTCCTCGACCATGATGTTATCTATCCCGTTTTCCGTGAACTTTTTCATAACCAAAATGCa  <  1:113848/251‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      ATCTGTTCCTCAGCAGAACGTATAAGAACGTGGAAGTCGTATACATCCGCCATCATACCCAGGCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCA  >  NC_000913/1869784‑1870223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑