Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,284 C→T D106D (GAC→GAT)  yehP → VWA domain‑containing protein YehP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,2840CT87.5% 13.1 / ‑6.2 9D106D (GAC→GAT) yehPVWA domain‑containing protein YehP
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (3/4);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCAC‑CCCTGAGTGGATCAACAGTATTCACACGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGATGTGGTGACGAATCTCGACGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGTACTC  >  NC_000913/2208075‑2208513
                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     
cAGATAGACCACGCGCTGGCGTGGCTGTATGGGCGCGATACTGAACGGATCCCGCGTGGTGAATATTAAGGTGGATTAGGAGGCACAAAACTC‑CCACACACTGAGCGGCTCAACAGTATTAACACGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGAGATGCCGTGCTGCGCTAAGACATTGAAGATGTGGTGACGAATCTCGATGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACgcgc                                                                                                                                                                                              >  1:111827/1‑251 (MQ=255)
                                                     gCGTGGTGAACGTTCCGGTGGCTTAGGTGGCTCAAATCTCACCAC‑CCCTGAGCGGATCACCCGTATTCACACGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGATGTGGTGACGAATCTCGATGTGCTGGAACGTATGCAGCCTTCTGAACGCCTGCTCCGCGCTGTTTTGCACACCAAACATCtgat                                                                                                                                                                      >  1:113030/1‑222 (MQ=255)
                                                                    cGGTGGATTAGGTGGCTCAAATCTCACCAC‑CCCTGAGTGGATCAACAGTATTCACCCGCTGTTTACGCAGCAGGAGATCGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGCTGTGGTGACGAATCTCGACGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCAAACCAAACATCTGATGAACCCCGACGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAgg                                                                                                                          >  1:114559/1‑251 (MQ=255)
                                                                     ggTGGATGAGGTTGCTCAAATCTCACCAC‑CCCTGAGTGGATCAACAGTATTCTCACGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGATGTGGTGACGAATCTCGATGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACAGCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGgt                                                                                                                         <  1:122297/251‑1 (MQ=255)
                                                                                                ac‑cCCTGAGTGGATCAACAGTATTCACACGCTGTTTCCGCAGCAGTTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGTCATTGAAGATGTGGTGACGAATCTCGATGTGCTGGAACGGATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACTCCAAACATCTTATGAACCCCGAAGTACTGGCTGCTTCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGc                                                                                              <  1:80825/251‑1 (MQ=255)
                                                                                                                         cacaCGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGATGTGGTGACGAATCTCGATGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCtttt                                                                        <  1:75261/249‑1 (MQ=255)
                                                                                                                             cGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGATGTGGTGACGAATCTCGATGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGGATGGCAAAGGAAGGTCGTCAGGCTTTTTCttgg                                                                  >  1:38571/1‑248 (MQ=255)
                                                                                                                                                                                             aGATGTGGTGACGAATCTCGATGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCTTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGTActc  <  1:1692/251‑1 (MQ=255)
                                                                                                                                                                                                        cGAATCTCGAGGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATGATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCa                                 <  1:154318/209‑1 (MQ=255)
                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     
CAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCAC‑CCCTGAGTGGATCAACAGTATTCACACGCTGTTTCCGCAGCAGGTGATTGAGCGGCTGGAAAGCGATGCCGTGCTGCGCTACGGCATTGAAGATGTGGTGACGAATCTCGACGTGCTGGAACGTATGCAGCCTTCTGAAAGCCTGCTACGCGCTGTTTTGCACACCAAACATCTGATGAACCCCGAAGTACTGGCTGCCGCCCGCCGGATAGTGTGCCAGGTTGTTGAAGAAATTATGGCTCGACTGGCAAAGGAAGTTCGTCAGGCTTTTTCTGGTGTCCGCGATCGCCGTCGCCGTTCATTTATTCCACTGGCGCGAAACTTTGATTTCAAAAGTACTC  >  NC_000913/2208075‑2208513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑