Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 542,098 G→A E71K (GAA→AAA)  glxK → glycerate 2‑kinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913542,0980GA87.5% 11.4 / ‑3.2 8E71K (GAA→AAA) glxKglycerate 2‑kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (1/6);  total (1/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

AATTTGTTGAAAAAGGATTGATAATGAAGATTGTCATTGCGCCAGACTCTTTTAAAGAGAGCTTAAGTGCAGAAAAATGTTGTCAGGCAATTAAAGCCGGGTTTTCGACCCTCTTTCCCGATGCGAACTATATCTGTTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTG  >  NC_000913/541865‑542338
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
aatTTGTTGAAAAAGGATTGATAATGAAGATTGTCATTGCGCCAGACTCTTTTAAAGAGAGCTTAAGTGCAGAAAAATGTTGTCAGGCAATTAAAGCCGGGGTTTCGACCCTCTTTCCCGATGCGAACTATATCTGTTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCAAAAAAGTGAATGCttt                                                                                                                                                                                                                                  <  1:164666/250‑1 (MQ=255)
                                                                                   cAGGCAATGAAAGCCGGGTTTTCGACCCTCTTCCCCGATGCGAACTATATCTCGTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCGAAAAAGTGAATGCTGTTTATGGCCTTACCGGCGACGTGAAAACGGCGGGGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCg                                                                                                                                              <  1:145209/251‑1 (MQ=255)
                                                                                        aaTTAAAGCCGGGTTTTCGACCCTCTTCCCCGATGCGAACTATATCTGTTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCAAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATc                                                                                                                                         <  1:72326/251‑1 (MQ=255)
                                                                                          ttAAAGCCGGGTTTTCGACCCTCTTTCCCGATGCGAACTATATCTGTTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCAAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTcg                                                                                                                                                           <  1:110842/231‑1 (MQ=255)
                                                                                                     ttttCGTCCCTCTTTCCCTATGCGAACTATATCTTTTTGCCGATGGCGTAGGGCGGCGAAGGGACGGTGGATGCGATGTTCGCCGCGACGGGCGGCAACATCTTAACGCTTGAAGTCTGCTGGCCGATGGGCAAAAAAGTGAATGCTTTTTATGGATTTACCGGCGACGGTATAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGa                                                                                                                                                    <  1:118947/227‑1 (MQ=255)
                                                                                                       ttCGACCCTCTTTCCCGATGCGAACTATATCTGTTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCAAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAAGCCGTTGCTGGCCTcn                                                                                                                           <  1:42998/250‑2 (MQ=255)
                                                                                                                                                                                                                    gAAGTCTGCGGGCCGATGGGCAAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGCCGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAACTGGCCCGAt                                                                                                                                                                   >  1:93518/1‑101 (MQ=255)
                                                                                                                                                                                                                               gCCGATGGGCAAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGAAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATTGTCATGGCGCAGGCGCTCGgtg  <  1:55706/251‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
AATTTGTTGAAAAAGGATTGATAATGAAGATTGTCATTGCGCCAGACTCTTTTAAAGAGAGCTTAAGTGCAGAAAAATGTTGTCAGGCAATTAAAGCCGGGTTTTCGACCCTCTTTCCCGATGCGAACTATATCTGTTTGCCGATAGCGGATGGCGGCGAAGGGACGGTGGATGCGATGGTCGCCGCGACGGGCGGCAACATCGTGACGCTTGAAGTCTGCGGGCCGATGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTG  >  NC_000913/541865‑542338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑