Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,849,728 G→A E298K (GAA→AAA)  sppA → protease IV, a signal peptide peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,849,7280GA87.5% 14.1 / NA 8E298K (GAA→AAA) sppAprotease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/4);  minor base C (1/0);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GCTTGAGGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTT  >  NC_000913/1849619‑1849973
                                                                                                             |                                                                                                                                                                                                                                                     
gCTTGAGGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCAATGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGgcgc                                                                                                                                    >  1:123022/1‑225 (MQ=255)
      gggTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGAAAACTCAgg                                                                                                          <  1:124135/245‑1 (MQ=255)
        ntttAAACAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGTTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAAAATTc                                                                                                                                                                                                                                                  >  1:41999/2‑107 (MQ=255)
                   aCCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTAGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGAAAACTCAGGGGAATGTTGGCGGTGATAc                                                                                       <  1:63694/251‑1 (MQ=255)
                          gCGATACCGAGAAATATGCACTGGAAAACAAGCTGGTAGATGCACTGGAATCGAGTGCGGAAATAGACCAAGCACTGACCACACAATTCGGCTGGAGTAAGACTGATACAACTTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGCAAACTCAGGGGAATGTTGGCGGTGATACCACTGCg                                                                                >  1:110029/1‑251 (MQ=255)
                                                                          cATCGAGGGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTTACATCATCGGTGTCGTCTTTGCTAATGGCGTAATTATGGAGGGTGAGAAAACGCAGGGGAATGTTGGCGGTGATAACTCTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGTGATTGTc                                <  1:23670/251‑1 (MQ=255)
                                                                                                 gCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCAGTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTatg                                                                                                                             <  1:81029/135‑1 (MQ=255)
                                                                                                        ccAAAAAATTCGGCTGGAGTACGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGCCTTTGCTAATGGCGCAATTATGGATGGCGCGAAAACTCAGGGGAATGTTGGCGGTGATACCCCTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGtt  >  1:63831/1‑251 (MQ=255)
                                                                                                             |                                                                                                                                                                                                                                                     
GCTTGAGGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTT  >  NC_000913/1849619‑1849973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: