Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,254,400	C→T	D263N (GAC→AAC)	rihB ←	pyrimidine‑specific ribonucleoside hydrolase RihB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,254,400	0	C	T	100.0%	16.1 / NA	8	D263N (GAC→AAC)	rihB	pyrimidine‑specific ribonucleoside hydrolase RihB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

CCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGG‑CTTGCCAAGAACGCCCA‑GCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGCCGTAGTTTTCGAACTGCGTTTTGAGAGTGAAGTTCATGATGTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGC  >  NC_000913/2254206‑2254624                                                                                                                                                                                                     |                                                                                                                                                                                                                                 ccTGCATCTTGCGATACCGGCGCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCAAGCACTCTTCGACTAATCCCCAGGACCAGTCTGTATCAATAGTGATGCCGACTTTGGGATTGGCGGGGCTT‑CCCCGAACGGCCACGCT‑GTCGCCGAAGGGTCCCCCCTGGCAAAGGCCACCTCTGACGTTCACTTCGGCGGACATCTCCCGGGTTTTAATTTCCTCTGGGTTTATCAGAAAACCg                                                                                                                                                                           >  1:35353/1‑250 (MQ=255)                                aTCAACGCTTAATGGGTTTTGATGTAGCCGCGCACGCACTCGCCGCCGAATCCCCAGAACCAGGCTGGATGAATAGTGAGGCCGACTTTGGTATTGGCGGG‑CTTGCCAAGAACGCCCA‑GCTCGTCGCAGACGGTACGCCCATAGCAGGGGCCAGGGTTTACGTTCACTTCTACGTACATCTCCGGGGGTTTAATCCCTTCAGGGTTAATGAAAGAACCGATGCTGGTGGCGTCGTGCACCGGGCCGCCAgn                                                                                                                                           <  1:15625/251‑2 (MQ=255)                                                                                                     tCAATAGTGATGCCGACTTTGGTATTGGCGGG‑CTTGCCAAGAACGCCCA‑GCTGGTCGCAGACGGTACGCCCAGAGCAAGGGCCGCTGGTTACGTTCACTTCGACGTACATCTCCTGGGTTTTAATGCCAGCAGGGTTAATCAGAGAACCGATGCAGGTGGCGTCGTGCCCCGGGCCGCCAGCAAGGCCGTAGTTTTCGAACTGCGTTTTg                                                                                                               <  1:74072/210‑1 (MQ=255)                                                                                                           gTGATGCCGACTTTGGTATTGGCGGG‑CTTGCCAAGAACGCCCC‑GCTCGTCGCAGACGGGACGCCCATAGCAAGGGCCACTGTTTACGTTCACTTCGACGTACATCTCCTGGGTTTTAATGCCCTCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGCCGTAGTTTTCGAACTGCGTTTTGAGAGGGAAGTTCATGATGTCGCTGAACAGCTCTCCGGCGGGgccgcc                                                                >  1:9806/1‑251 (MQ=255)                                                                                                                          gTATTGGCGGG‑CTTGCCAAGAACGCCCA‑GCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTTCACTTCGACGTACATCTCCTGGGGTTTAATGCCATCAGGGTTAATCAGAGAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGCCGTAGTTTTCGAACTGCGTTTTGAGAGTGAAGTTCATGATGTCGCTGAACAGCGCTCCGGCGGGGCCGCCTGACCTTTCCATCCg                                                 <  1:108518/251‑1 (MQ=255)                                                                                                                                          ccAAGNACGCCNA‑NNTCGTCGCAGACGGTACGCCNATAGCAAGGGCCACTNTTGANGTTCANtg                                                                                                                                                                                                                             >  2:71079/1‑63 (MQ=255)                                                                                                                                          ccAAGAACGCCCA‑GCTCGTCGCAGACGGTACGCCCATAGCAAGTGCCACTGTTGACGTTCACTTCGACGTACATCTCCTGGGTGTTAATTCCATCAGGGTTAATCAGATAACCGATGCAGGTGGc                                                                                                                                                                <  1:71079/125‑1 (MQ=255)                                                                                                                                                                           cccATAGCAAGGGCCACTGTTGACGCTCACTTCGACGTACCTCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCCGCCAGGCCGTAGTTTTCGAACTGCGTTTTGAGAGTGAAGTTCATGATGTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCCTCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGc  >  1:136213/1‑251 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                                 CCTGCATCTTGCGATACCGGCCCGGCTTGTTATCAACACTTAATGGGTTTTGATGTAGCCGCGCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGG‑CTTGCCAAGAACGCCCA‑GCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATCAGGGTTAATCAGATAACCGATGCAGGTGGCGTCGTGCACCGGGCCGCCAGCAAGGCCGTAGTTTTCGAACTGCGTTTTGAGAGTGAAGTTCATGATGTCGCTGAACAGCTCTCCGGCGGGGCCGCCTGCCCTTTCCATCCGAGCAATCACGTCCGGGGTGCAAACGGTCTGGTTGGTGAGATCGAGGC  >  NC_000913/2254206‑2254624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑