Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,257,367	C→T	intergenic (‑32/+62)	psuT ← / ← psuG	putative pseudouridine transporter/pseudouridine‑5'‑phosphate glycosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,257,367	0	C	T	100.0%	15.3 / NA	7	intergenic (‑32/+62)	psuT/psuG	putative pseudouridine transporter/pseudouridine‑5'‑phosphate glycosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

TCACTGTAAGACATCACTTTATGAACGCCTAATGCCGCCTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTC  >  NC_000913/2257124‑2257596                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      ncACTGTAAGACATCACTTTATGAACGCCTAATGCCGCCTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGTGTCAgcg                                                                                                                                                                                                                                >  1:18282/2‑251 (MQ=255)                                                            cGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGTGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTaan                                                                                                                                                                     <  1:100816/251‑2 (MQ=255)                                                                         agCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGTGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGCCAGAAATTAACCCGCGAGACGCTg                                                                                                                                                        <  1:994/251‑1 (MQ=255)                                                                                    accaATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCGTTATATCCAGAAGGATTTTCCCTTCATGCCGGTAAACCCGGTGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTgg                                                                                                                                             <  1:25567/251‑1 (MQ=255)                                                                                                                                             tttATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGTGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAgg                                                                                     <  1:57921/250‑1 (MQ=255)                                                                                                                                                                          ccAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGTGTCAGCGCCAGGTTTTGGAATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAACTTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGCATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAAcgcgc                                                       >  1:111052/1‑251 (MQ=255)                                                                                                                                                                                                                               ccTTCATGCCGGTAAACCCGCTGTCAGCGCCAGGATTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGagcttcagcttc  >  1:144229/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      TCACTGTAAGACATCACTTTATGAACGCCTAATGCCGCCTGTTCTACTGCCCATTTTCCCGGTGGGAAGTAGAGCATGATGCCACCAATAGCGATTTGCAGCAGCAGTGCGGCTCCAACCGTGCGCAAACTGATGCTCTTTTTATTCACTGACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCTTCAGCTTC  >  NC_000913/2257124‑2257596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑