Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,929,123	C→T	E161K (GAA→AAA)	viaA ←	protein ViaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,929,123	0	C	T	100.0%	13.6 / NA	8	E161K (GAA→AAA)	viaA	protein ViaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/5);  total (3/5)

CCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTGTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTCCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTAATAGCTGTTGATTTAACGTCGTTGCTTGCACGATCAGACTTAAACGCCAACGCTGGAGAAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGG  >  NC_000913/3928898‑3929274                                                                                                                                                                                                                                  |                                                                                                                                                        ccATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGGTGCAGTTCCGGCTGTTCGTTAAGAAATTCACCGGATTTTACAATCAACTGAGAGTCGCCACGTTTAAGCTGACCGTCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGTATCGGTTCAAGGTGTCCGCTCAGCGTCATGCGTTTCTGAACTTCACTCAACAGTTGTTcg                                                                                                                                <  1:171871/251‑1 (MQ=255)       gcgcATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTGTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTTCTGAACTTCACTCAACAGTTGTTCGCGttct                                                                                                                          <  1:96419/251‑1 (MQ=255)                tGCGCGGTATTGATTTGGCTGCCCGAGAACTCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTGTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCTCGTTTAAGCTGACCGGCGCTCATATCCCACATACGACCAGCTGCAGTATTGGTATCTGCGATAATCGGTGCAAGGTGTCCGCTCAGGGTCATGCGGTTCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTaa                                                                                                                  <  1:67622/250‑1 (MQ=255)                    cgGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTATGCCAGGCGTTTCAGTTCCGGCTGTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTTCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTAATAGCt                                                                                                             >  1:72121/1‑251 (MQ=255)                                                              cAGGCGTTTCAGTTCCGGCTGTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTTCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTAATAGCTGTTGATTTAACGTCGTTGCTTGCACGATCan                                                                              <  1:101463/240‑2 (MQ=255)                                                                                  gTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGGCCGCTCAGCGTCATGCGTTTCTGAACTTCTCTCAACAGTTGTGCGCGTGCTTCTGCTAATAGCGATTGATTTAACGTCGTTGCTTGCACGATCAGACTTAAACGCCAACGCTGGAGAAAAAGCgtg                                               <  1:148882/251‑1 (MQ=255)                                                                                                                 cAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTTCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTAATAGCTGTTGATTTAACGTCGTTGCTTGCACGATCAGACTTAAACGCCAACGCTGGAGAAAAAGCGTGTGTAACGCTGAAGTGATCg                            >  1:55930/1‑239 (MQ=255)                                                                                                                               naCGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTTCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTAATAGCTGTTGATTTAACGTCGTTGCTTGCACGATCAGACTTAAACGCCAACGCTGGAGAAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCgg  >  1:18149/2‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                        CCATCTGCGCATCGTTGCGCGGTATTGATTTGGCTTCCCGAGAACGCCCCAGCTGCTCTGCCAGGCGTTTCAGTTCCGGCTGTTCGTTAAGAAATTCACCGTATTTCACAATCAACTGATAGTCGCCACGTTTAAGCTGACCGGCGCTCATATCCCACAGACGACCAGCTGCAGTATTGTTATCTGCGAGAATCGGTTCAAGTTGTCCGCTCAGCGTCATGCGTTCCTGAACTTCACTCAACAGTTGTTCGCGTTCTTCTTCTAATAGCTGTTGATTTAACGTCGTTGCTTGCACGATCAGACTTAAACGCCAACGCTGGAGAAAAAGCGTGTGTAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGG  >  NC_000913/3928898‑3929274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑