Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	178,317	C→T	S49N (AGC→AAC)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	178,317	0	C	T	100.0%	24.8 / NA	10	S49N (AGC→AAC)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

AGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGATCGGGTTTCAGCGCGACAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCTCAATCTTTTGCGCTTGTGGAGGATAGTCGGAATAGCTGCTGACCCCAACCGGCGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCGGGAGAAAGCGTGATGACGCGCGGCGCGGCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTG  >  NC_000913/178090‑178550                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          aGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAAGCACCAGATCGGGTTTCAGCGCGACAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCGCAATCTGTTGCGCTTGTGGAGGATAGTCGGAATAGTTGCTGACCCCAACCGGTGTGATc                                                                                                                                                                                                                    <  1:94180/251‑1 (MQ=255)                                                    cGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGACAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGAGCGGGTGTCAGCGCGACAAGGCTGTCCAGAGTCATCCCCGGCCAGGTGGAAACCTGCTCAATCTTTTGCGCATGTGGAGGATAGTCGGAATAGTTGCTGACCCCAACCGGTGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCTGGAGAAAGCGTGATGAcgcgc                                                                                                                                                                 <  1:110686/251‑1 (MQ=255)                                                               ngtnngaatNCCAGCGAGGCCTGCGGGTCAACCGNCCGCTCGGCATTACCTCCGCGCCAGGCAATCNCNAGATCGGGTTTCAGCGCGACAATGCGTTCCAGAGTCATCCCCTGCCAGNTGGAAACCTGNTCANNCTGTNGNGNTTGTGGATGATAGTCGGAATAGTTGCTGANCCCAACCGGTGTGATCCCGGCGGNNAANNNAANNTCAgc                                                                                                                                                                                             <  2:127601/204‑2 (MQ=255)                                                                         ccAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGAAATCACCAGATCGGGTTTCAGCGCGCCAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCTCAATCTTTTGCGCTTGTGGAGGATAGTCGGAATAGTTGCTGACCCCAACCGGTGTGATCCCGGCGGCAAAGGCACGTTCAgc                                                                                                                                                                                             >  1:127601/1‑201 (MQ=255)                                                                                             aCCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGCTCGGGTTTCAGCGCGACAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCTCAATCTTTTGCGCTTGTGGCGGATAGTCGGAATAGTTGCTGAcc                                                                                                                                                                                                                                   >  1:52780/1‑144 (MQ=255)                                                                                                                          gtcAATCACCAGATCGGGTTTCAGCGCGACAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCTCAATCTTTTGCGCTTGTGGAGGATAGTCGGAATAGTTGCTGACCCCAACCGGTGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCGGGAGAAAGCGTGATGACGCGCGGCGCGGCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGcc                                                                                            >  1:161724/3‑250 (MQ=255)                                                                                                                                 aCCAGATCGGGTTTCAGCGCGACAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCTCAATCTTTTGCGCTTGTGGAGGATAGTCGGAATAGTTGCTGACCCCAACCGGTGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCGGGAGAAAGCGTGATGACGCGCGGCGCGGCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCa                                                                                     >  1:60696/1‑250 (MQ=255)                                                                                                                                                                                                                  ggaggaTAGGCGGAATAGTTGCTGACCCCAACCGGTGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCGGGAGAAAGCGTGATGACGCGCGGCGCGGCGTTGAGCCACAGTGGCGCAAGAAAAGACAGTGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCATCAGCCAGGAACTCATCGAAGCTAAGATGAGACt   <  1:151413/251‑1 (MQ=255)                                                                                                                                                                                                                   gaggaTAGTCGGAATAGTTGCTGACCCCAACCGGTGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCGGGAGAAAGCGTGATGACGCGCGGCGCGGCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTg  >  1:118871/1‑251 (MQ=255)                                                                                                                                                                                                                   gaggaTAGTCGGAATAGTTGCTGACCCCAACCGGGGAGATCCCGGCGGCAAAGGCAAGTTCAATGTTGGCGGGAGAAAGAGTGATGACGCGCGGCGCGGCTTTGAGCCACAGTGGCGCACGAAAAGACAGGACGACCAGCGCCCTGAACAGTGACTTAACCATGTGCAAGTTTCTGCACCAGTGACTCTACCATCAGGCTGGACTGATAAGCGGCAACAGCCAGGACCTCATCGACGATAAGATGAGAcgg  >  1:118866/1‑249 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          AGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTCAATGCTTGTCGCATCGACCCACATCACTTTTATTCCCAGCGAAGCCAGCTGGTCAACCTGCCGCTCGGCATTACCTCCACGCCAGGCAATCACCAGATCGGGTTTCAGCGCGACAATGCGTTCCAGATTCATCCCCTGCCAGGTGGAAACCTGCTCAATCTTTTGCGCTTGTGGAGGATAGTCGGAATAGCTGCTGACCCCAACCGGCGTGATCCCGGCGGCAAAGGCAAGTTCAGTGTTGGCGGGAGAAAGCGTGATGACGCGCGGCGCGGCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTG  >  NC_000913/178090‑178550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑