Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,455	C→T	G319D (GGT→GAT)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,455	0	C	T	100.0%	24.1 / NA	11	G319D (GGT→GAT)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/2);  total (9/2)

GCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACG  >  NC_000913/2260224‑2260688                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          ncccccAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCCGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATATCACCAATGAAGCCAGAAcc                                                                                                                                                                                                                        >  1:31604/2‑251 (MQ=255)               cGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCCACTGGTTTACCGATCAGGTAGCTCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCCGGAAGCCCGCAATAATATCCCCAATGAAGCCAGAACCGGTGCTGACCGcc                                                                                                                                                                                                           >  1:71204/1‑250 (MQ=255)                     cGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCATTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTACCTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATATCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATAc                                                                                                                                                                                                    >  1:107074/1‑251 (MQ=255)                            cccATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGTTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTTGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATCTCCCCAAAGAAGCCAGCACCGGTTCTGCCCGCCAGCCTACCGcccat                                                                                                                                                                                             >  1:13747/1‑248 (MQ=255)                                                                                                catcGCCAGACCGACCAACCGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTAGCAATGAAACCAGCCAGGAAGCCCGCAATAATATCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCAcc                                                                                                                          >  1:47065/1‑250 (MQ=255)                                                                                                                        ggAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATATCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAaccacc                                                                                                   <  1:50400/249‑1 (MQ=255)                                                                                                                               aGCGGAATGATCAGGATCGGTTTCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATATCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTg                                                                                          <  1:137417/251‑1 (MQ=255)                                                                                                                                                     tCAGCGCTTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATATCACCAATGAAGCCAGAACCGGTGCTGACCGCCAg                                                                                                                                                                                                         >  1:53065/1‑118 (MQ=255)                                                                                                                                                                                                       gCAATGTAACCAGCCAGGAAGCCCGCAATAATATCACAAATGAAGCCAGAACCGGTGATGACCGCCAGAATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGAtg                                                                                                                                                                  >  1:164341/1‑106 (MQ=255)                                                                                                                                                                                                                    ccagGAAGCCCGCAATAATCTCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCCATATAACCTGCCAGTACCgg                                                                                                                              >  1:138849/1‑130 (MQ=255)                                                                                                                                                                                                                        gAAGCCCGCAATAATATCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACg  >  1:6725/1‑250 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          GCCCCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACG  >  NC_000913/2260224‑2260688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑