Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,861,253	C→T	intergenic (+77/+734)	yidL → / ← glvB	putative DNA‑binding transcriptional regulator YidL/putative PTS enzyme II component GlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,861,253	0	C	T	100.0%	10.2 / NA	5	intergenic (+77/+734)	yidL/glvB	putative DNA‑binding transcriptional regulator YidL/putative PTS enzyme II component GlvB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/2);  total (3/2)

CATTTCAGCAAAGCCTTTAAACATAAATTTGGTTATGCGCCGTCAGCCGTGCTAAAGAATACGGACCAGCACCCAACGGATGCCAGTCCACACAATTAACGTAACTCTGGCCAGTACGCTTTGTTGGCCTCCAGCAACTCATCCAGAATAGCTTTGGCGACCGAGGCGCTCGGTACCGTTTTCGACAACGTAATCGCCTGCCACAGGTGCTGATATGAACGCTGTTCCCAGGCGTCCACGACCAGTTTTTCCACCGCCACTTGCTGACTCATTAACCCTTTCTGAAACTGCGGGATATCGCCGACCACCAGTGGTTCAGGCCGTAGTAGCGCACGCGCATCTGTTTGCGATCTTGCAGCCCAACAATTTGCGCCATCCGGCTTTCAATACCGATTGGCATGTCACAGATGTTGAGGATT  >  NC_000913/3861078‑3861496                                                                                                                                                                                |                                                                                                                                                                                                                                                    catttcaGCAAAGCCTTTAAACATAAATTTGGTTATGCGCCGTCAGCCGTGCTAAAGAATACGGACCAGCACCCAACGGATGCCAGTCCACACAATTAACGTAACTCTGGCCAGTACGCTTTGTTGGCCTCCAGCAACTCATCCAGAATAGCTTTGGCGACCGAGGCGCTCGGTATCGTTTTCGACAACGTAATCGCCTGCCACAGGTGCTGATATGAACGCTGTTCCCAGGCGTCCACGACCAGtttttn                                                                                                                                                                          <  1:40296/251‑2 (MQ=255)                                     gcgcCGTCAGCCGTGCTAAAGAATACGGACCAGCACCCAACGGATGCCAGTCCACACAATTAACGTATCTCTTGCCGGTACGCTTTGTTGGCCTCCAGCAACTCATCCAGAATAGCTTTGTCTCCCGAGGCGCTCGGTATCGTTTTCGACAACGTAATCGCCTGCCACAGGTTCTTATATGAACGCTTTTTCCAGGCGTCCACGTCCATTTTTTCCTCTTCCACTTTCTGACTCATTAACCCTTTCTGaa                                                                                                                                       <  1:72117/250‑1 (MQ=255)                                                                                         cacacaATTAACGTAACTCTGGCCAGTACGCTTTGTTGGCCTCCAGCAACTCATCCAGAATAGCTTTGGCGACCGAGGCGCTCGGTATCGTTTTCGACAACGTAATCGCCTGCCACAGGTGCTGATATGAACGCTGTTCCCAGGCGTCCACGACCAGTTTTTCCACCGCCACTTGCTGACTCATTAACCCTTTCTGAAACTGCGGGATATCGCCGACCACCAGTGGTTCAGGCCGTAGTAGCGCACgcgc                                                                                   >  1:54350/1‑250 (MQ=255)                                                                                                               ccAGTACGCTTTGTTGGCCTCCAGCAACTCATCCAGAATAGCTTTGGCGACCGAGGCGCTCGGTATCGTTTTCGACAACGTAATCGCCTGCCACAGGTGCTGATATGAACGCTGTTCCCAGGCGTCCACGACCAGTTTTTCCACCGCCACTTGCTGACTCATTAACCCTTTCTGAAACTGCGGGATATCGCCGACCACCAGTGGTTCAGGCCGTAGTAGCGCACGCGCATCTGTTTGTGATCTTGCAGccc                                                            >  1:148621/1‑251 (MQ=255)                                                                                                                                                                         ntCGGTATCGTTTTCGACAACGTAATCGCCTGCCACAGGCGCTGATATGAACGCTGTTCCCAGGCGTCCACGAACAGTTTTTCCACCGACACTTGCTGACTCATTAACCCTTTCTGAAACTGCGCGATATCGCCGCCCACCCGTGGTTAAGGCCGTAGTAGCGCACGCGCATCTGTTTGCGATCTTGAAGCCCAACAATTTGCGCCATCCGGCTTTCAATACCGATTGGCATGTCACAGATGTTGAGGAtt  >  1:15139/2‑251 (MQ=255)                                                                                                                                                                                |                                                                                                                                                                                                                                                    CATTTCAGCAAAGCCTTTAAACATAAATTTGGTTATGCGCCGTCAGCCGTGCTAAAGAATACGGACCAGCACCCAACGGATGCCAGTCCACACAATTAACGTAACTCTGGCCAGTACGCTTTGTTGGCCTCCAGCAACTCATCCAGAATAGCTTTGGCGACCGAGGCGCTCGGTACCGTTTTCGACAACGTAATCGCCTGCCACAGGTGCTGATATGAACGCTGTTCCCAGGCGTCCACGACCAGTTTTTCCACCGCCACTTGCTGACTCATTAACCCTTTCTGAAACTGCGGGATATCGCCGACCACCAGTGGTTCAGGCCGTAGTAGCGCACGCGCATCTGTTTGCGATCTTGCAGCCCAACAATTTGCGCCATCCGGCTTTCAATACCGATTGGCATGTCACAGATGTTGAGGATT  >  NC_000913/3861078‑3861496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑