Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,345	G→A	N26N (AAC→AAT)	ibpA ←	small heat shock protein IbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,345	0	G	A	100.0%	17.7 / NA	8	N26N (AAC→AAT)	ibpA	small heat shock protein IbpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

CTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGT  >  NC_000913/3867114‑3867580                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            ctcAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTAAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGATTGTTTTCTAAGTGGTTa                                                                                                                                                                                                                           >  1:10889/1‑250 (MQ=255)                                       cGATGCCCTGGTAAAGATAGGTGCGCTCTTTTTGTTCGTCTGCGGGAGCCCCTTTCACCTCCAGCAGATTAGCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTTATGGTTTTCGTCTACCAGTGCAACGTTATACGGAGGTTAGCCGCCATTACTCTGGCTCTGATTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCggg                                                                                                                                                                                    <  1:69349/251‑1 (MQ=255)                                             ccTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGCGCACCTTTCACCACCAGCAGGTTATCCTGGGCGGTCATTTCCAGTTCGCTCTCATCAACACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCCGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGATTGTTTTCTAAGTGGTTAAACAAACGGTCAATTCCAATAGCAGACCGGTAAAGCGGGGATaa                                                                                                                                                                               >  1:54313/1‑250 (MQ=255)                                             ccTGATACAGATAGGTGCGCTCTTTTTGTTCGGCGGCGTGAGCCCCTTTCACCCCCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCATCAAAACCAGCCACAGCGATAGCAATGCTGTAATGGTTTTCGTCTACCATTTCAATTTTATACGAAGGGTAGCCGCCATTACTCTGGCTCTGATTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAg                                                                                                                                                                                                  <  1:25894/231‑1 (MQ=255)                                                    cAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCCCCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGATTGTTTTCTAAGTGGTTAAACAAACGGTCAAAGCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGt                                                                                                                                                                       <  1:50598/251‑1 (MQ=255)                                                                                                                                           aaaaaacccAGCCACCGCGATAGCAATGCGGTAATGGTTTTCCTCTCCCAGTTCAACGTTATCCTGAGGGTCGCCGCCATTACTCTGGCTCTGATTGTTTTCTAAGTTGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGTGGATAAATCAAAGTTTCGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGgcg                                                                                <  1:5246/244‑1 (MQ=255)                                                                                                                                                                                                                        cATTACTCTGGCTCTGATTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGAc                                                                                                                            >  1:96008/1‑130 (MQ=255)                                                                                                                                                                                                                         aTTACTCTGGCTCTGATTGTTTTCTAAGTGGTTAAACAAACGGTCAAATACAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGt  >  1:63214/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                            CTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAACGTTATACGGAGGGTAGCCGCCATTACTCTGGCTCTGGTTGTTTTCTAAGTGGTTAAACAAACGGTCAAATCCAATAGCAGAACGGTAAAGCGGGGATAAATCAAAGTTACGCATAATCAATAGCTCCTGAAATCAGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGT  >  NC_000913/3867114‑3867580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑