Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,009,059	C→T	I261I (ATC→ATT)	yedE →	putative selenium transporter YedE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,009,059	0	C	T	100.0%	14.4 / NA	7	I261I (ATC→ATT)	yedE	putative selenium transporter YedE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

CAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGTCTGGGCAATGTG  >  NC_000913/2008917‑2009269                                                                                                                                               |                                                                                                                                                                                                                   caGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATTATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGATACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACtn                                                                                                        <  1:41336/251‑2 (MQ=255)                               cAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACATGCGCAAATCTGCTTTACTTCAGCGTTCAGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATTATTATCGGTATGGCGGAGAGTGCCAACGGGAGCTTCAGTTACGTACCGTTAGTCGTTGTCCCCCAAATCATGAGGGCGGGAAAAAACCCCGGAATTGGTGGGGTACTGTTTggggatcggaattgccaacgcctgaa                                                                           >  1:110705/1‑226 (MQ=255)                                                          tACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATTATTATCGGTATGGCGGTGAGTGCCATCGGGGTCTTTAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGGCCAAACGCGGGAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGGTGGCGGCTGCGAAACCGGCTGGATGTAc                                                     >  1:50804/1‑245 (MQ=255)                                                                                   cTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATTATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTan                                                     <  1:99202/220‑2 (MQ=255)                                                                                   cTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATTATTATCGGTATGGCGGGGAGTGCCAACCGGATCTTCCCTTTCGTACAGTTTGGGGTTTAAACCAAAAACATGNgggnnnnngcnn                                                                                                                               >  2:99202/1‑137 (MQ=255)                                                                                                                 cACCGGACGTACCCATATGGCGAAAGCAATTATTATCGGTATGGCGGTGAGTGCTATCGGGATCTTTAGTTTCGTACAGTTAGGCGTTGAACCCCAAATCATGTGGGGGGGGCCCAACGCGGGAATTGGTGGGTTACTGTTTTGTTTTGGCATCGTGCTGGCTGGCGGCTGCGACACCGGCTGGAGGTCCCGCGCGGTAGAAGGCCAGGGGCACTACTGGTGGGTCGGGCTGGGCAAtgtg  >  1:115792/1‑241 (MQ=255)                                                                                                                                          gCAATTATTATCGGTATGGCGGTGAGTGCCATCGGGAGCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTg                                                                        <  1:91987/146‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                                                                                   CAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAAACCGGCTGGATGTACCGCGCGGTAGAAGGCCAGGTGCACTACTGGTGGGTCGGTCTGGGCAATGTG  >  NC_000913/2008917‑2009269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑