Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,565,649	G→A	D412D (GAC→GAT)	eutA ←	ethanolamine ammonia‑lyase reactivase EutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,565,649	0	G	A	100.0%	13.5 / NA	7	D412D (GAC→GAT)	eutA	ethanolamine ammonia‑lyase reactivase EutA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

TACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGCAGTTG  >  NC_000913/2565428‑2565888                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                aaactgatatacattgccgaacaatgtggtctttagtttcataagtcgttccctcaggaagGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAATCCTGCCCGGCCACCACCAGCAGGGGATg                                                                                                                                                                                                                    <  1:170723‑M2/190‑1 (MQ=255)                                    gtttcataagtcgttccctcaggaagGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAATCCTGCCCg                                                                                                                                                                                                                                        <  1:12730‑M2/170‑1 (MQ=255)                                                                                                                ggCGTAGCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGGTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAATCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGTAAAACGCGCGACTAAATCGACCAACGCTTTGATGACCGTCAGTACCGCGGCGTAATGCACATGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGtttt                                                                                                     <  1:160486/251‑1 (MQ=255)                                                                                                                                                naCAATCACTTCGTCAATGACTGCCAACGGGCGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAACATCCTGCCCGGCCACCACCCGCAGGGGATGCGGATTCGGAAAACGCGCGCCGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGAGCCCGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt                                                                     >  1:19137/2‑251 (MQ=255)                                                                                                                                                          tCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAATCCTGCCCGGCCCCCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCCGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGGTGCACGGcccc                                                           >  1:88390/1‑251 (MQ=255)                                                                                                                                                                                                      caacaTGCCCAGAGCTTTACCAAAATCCTGCCCGACCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAATCGCCAGCACGCACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTTTTGCCAGGCCACCACCAGATCCGTTTCATCAATCGTGATCGCCACCGGCAAATTgc               >  1:66630/1‑251 (MQ=255)                                                                                                                                                                                                                   gCTTTACCAAAATCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGGACGCGTCTGTTTTGGGATCAAGATCCAGCTGAATCAGCTCCTGATGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGTAGTTg  <  1:84196/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                TACAATTCCACCAGCACCCAGCCACCCAGCGCCACCGCTGGCGAATACCGCATTCAGGAAGGAAATGCGAGTGATTTCACCGTCACCGGCACAACCGATCCGCCAAAAAGAGGCGTACCAATGTCGATATAGTCCCCCGCGCGGACAATCACTTCGTCAATGACTGCCAACGGGAGTTGTTGTAGCTGTGGGCGCAACAACATGCCCAGAGCTTTACCAAAGTCCTGCCCGGCCACCACCAGCAGGGGATGCGGATTCGGAAAACGCGCGACGAAATCGACCAACGCGTTGATGACCGTCAGTACCGCGGCGTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCAAATTGCGCAGCGGCAGTTG  >  NC_000913/2565428‑2565888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑