Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,760,546	G→A	Q416* (CAA→TAA)	sufD ←	Fe‑S cluster scaffold complex subunit SufD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,760,546	0	G	A	100.0%	10.2 / NA	6	Q416* (CAA→TAA)	sufD	Fe‑S cluster scaffold complex subunit SufD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

AACTCGGCCTCGGCGTCAATCACCTGGCTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTT  >  NC_000913/1760387‑1760777                                                                                                                                                                |                                                                                                                                                                                                                                        aaCTCGGCCTCGGCGTCAATCGCCTGGCTCGGTTTCTGCGCACTGGCGGCGCTTTCGAGATAAGCCAGCAGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAAGCATCTTGCACCTCCTGGCAGCCGTTAACCGATGCGGGCCAGCACCTGCGGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCCTCTGCTgg                                                                                                                                              <  1:96892/251‑1 (MQ=255)             gcGTCAATCACCTGGCTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCCGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGGCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTAACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCctgctg                                                                                                                                   >  1:121042/1‑250 (MQ=255)                                                                         aaaCCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTAACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTcgcg                                                                      <  1:140433/251‑1 (MQ=255)                                                                         aaaCCGTTTACCTCACGCGAAAGAACCGGAAAGTCGGCCCGCACTTTGTCGACCGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTAACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTcgcg                                                                      >  1:63650/1‑251 (MQ=255)                                                                                                                                             gCACCTCCTGGCAGCCGTTAACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGttt  <  1:161942/251‑1 (MQ=255)                                                                                                                                                            cGTTAACCGAGTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTACGGCAGCGAAGGCGTAAATGATCATCGGCTGTGCATCCTGCTGAATGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCgg                          <  1:165685/212‑1 (MQ=255)                                                                                                                                                                |                                                                                                                                                                                                                                        AACTCGGCCTCGGCGTCAATCACCTGGCTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTT  >  NC_000913/1760387‑1760777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑