Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,760	C→T	G339D (GGT→GAT)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,760	0	C	T	100.0%	20.1 / NA	9	G339D (GGT→GAT)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/7);  total (2/7)

ATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAACTTCGTTCAGCTCGTGATCACCGCGCACCAGCAGCGCAACCTGCGGGAAGCTGCTGCCTTCAACCGCTTTAACCAGCAGAGTCTTAACCGTTTTC  >  NC_000913/217562‑217951                                                                                                                                                                                                       |                                                                                                                                                                                                aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCGCGGCACGAAGTGCTTCTTCGGTcgc                                                                                                                                             <  1:51866/251‑1 (MQ=255)                                     ncagccaCCCCGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCACGGCACGACTTTCTTCTTCGGTcgc                                                                                                                                             >  1:48215/2‑215 (MQ=255)                                                     ggATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAACTTCGTTc                                                                                         <  1:50305/251‑1 (MQ=255)                                                                                          ccAGTTGATGCCGAAGTAGTGTTTACCAGCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGTCCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGt                                                                                                                                                                                            <  1:64540/115‑1 (MQ=255)                                                                                                    ccGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTa                                                                                                                                                                          <  1:6793/123‑1 (MQ=255)                                                                                                      gAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAACTTCGTTCAGCTCGTGAGCACCGCGCACCAGCAGCGCAACCTGCGGGAAGCtgctgc                                        <  1:133028/251‑1 (MQ=255)                                                                                                                                            aaTCACGCAGCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTTCGGCAGTTTTTCTGCTTTAACTTCGTTCAGCTCTTGAGCACCGCGCACCAGCATCGCTACCGGCGTGAAGCTGCTGCCTTCAACCTCTTTAACCAGCAGAGTCTTAACCGTTTtc  <  1:112025/251‑1 (MQ=255)                                                                                                                                                   naTCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCACGGCAc                                                                                                                                                                >  1:100087/2‑86 (MQ=255)                                                                                                                                                   cATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGATCCAGTGAACCCGGACCGGCTTTAACCACGGCAc                                                                                                                                                                <  2:100087/86‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                ATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGGCATGTTTACCGGACCCAGTGAACCCGGACCGGCTTTAACCACGGCACGAATTTCTTCTTCGGTCGCGAAAGTCAGCGGGCTTGCAACCTGCGGCAGTTTTTCTGCTTTAACTTCGTTCAGCTCGTGATCACCGCGCACCAGCAGCGCAACCTGCGGGAAGCTGCTGCCTTCAACCGCTTTAACCAGCAGAGTCTTAACCGTTTTC  >  NC_000913/217562‑217951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑