Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,673	G→A	E93E (GAG→GAA)	ydiS →	putative electron transfer flavoprotein‑quinone oxidoreductase YdiS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,673	0	G	A	100.0%	35.4 / NA	14	E93E (GAG→GAA)	ydiS	putative electron transfer flavoprotein‑quinone oxidoreductase YdiS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

ATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGC  >  NC_000913/1781464‑1781895                                                                                                                                                                                                                  |                                                                                                                                                                                                                               aTGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTCAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCCATCATTCCAGGCTTTGCAGTATACGCGCCGGTCGAACGCAAGGGCACACGCCAAAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTAGATTTTCACCGCGAACAGCCAGATGGTCCGCAACCAGCATCTTATACCGGATTGCg                                                                                                                                                                                       >  1:154685/1‑251 (MQ=255)                              gATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCATCTTATACCGTAGTGCGTAAGCGTCTg                                                                                                                                                                             <  1:134375/232‑1 (MQ=255)                                 aGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGGCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAAACGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCCTCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACaa                                                                                                                                                        >  1:12672/1‑250 (MQ=255)                                                tGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCACACACACTTGAAGCAATCATTACAGGCTTTGCAGTATCAGCGCCGGTAGCACGAAAGGTCACACGCGAGAACATCTCCTTCTTAAACGCAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTATGGACCCATGGGTGATGGAACAAGCCGAGCAggcgggcg                                                                                                                                        >  1:92938/1‑251 (MQ=255)                                                            aaaCATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTGCCGCAACACTCATCTTATATCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATcc                                                                                                                            <  1:24379/251‑1 (MQ=255)                                                                          ggcgTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAGCGCAATGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTc                                                                                                              <  1:38829/251‑1 (MQ=255)                                                                           gcgTCTTTATGCCCACACACTTGAAGCACTCATTCCAGGCTATGCAGTATCAGCGCCGGTAGAACGCAAGGTCCCACGCGAGAAAATCTCCTTCTTCAACGAAGAAAGCGCCGTTACACTCCATTTTCACCGCGAAAAGCCCGATGTTCCGCAACACGCATCTTATACCGCATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACCAGCCGAGCAGGCGGGCGCGCAGTTTATCCCCGGCGTTCGCGTCg                                                                                                             >  1:73959/1‑251 (MQ=255)                                                                                                   aGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTATAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGCACAGCCAGATGTTCCGCAACACNCATNNNNNAcnn                                                                                                                                                                                              >  2:99116/1‑144 (MQ=255)                                                                                                                 gcttggcaggagcAGCGCCGGTAGAACGCAATGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGAGTGGCACCGCGAACAGCCGGATGTTCCGTAACACGCATCTTATACCGTATTGCGTAATCGTCTGGAcn                                                                                                                                                                         <  1:19340/141‑2 (MQ=255)                                                                                                                                         naCGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGACAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCACTGGTTCGTGAAGGAAACACGGACACTGGCGGGCAGGGTGGGGATGATATTCTCg                                                 >  1:20521/2‑249 (MQ=255)                                                                                                                                          aCGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTAGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGG‑ATGATATTCTCGAAGc                                             <  1:98123/251‑1 (MQ=255)                                                                                                                                                                               gaagaaAGCGCCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGAAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGGTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGAGATTCTGGCTGATGGCGTTAACTCGAt         >  1:173310/1‑251 (MQ=255)                                                                                                                                                                                      gcgcCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGTAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATTCTTGGc  <  1:133803/251‑1 (MQ=255)                                                                                                                                                                                      gcgcCGTTACCCTCGATTTTCACCGCGAACAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTTTGGCTGATGGCGTTAACTCGATGCTTGGc  >  1:150196/1‑251 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                               ATGGCACGAGCCGGGCTGGATGTCCTGGTGATAGAACGCGGCGACAGTGCCGGATGTAAAAACATGACCGGCGGGCGTCTTTATGCCCACACACTTGAAGCAATCATTCCAGGCTTTGCAGTATCAGCGCCGGTAGAACGCAAGGTCACACGCGAGAAAATCTCCTTCTTAACCGAAGAAAGCGCCGTTACCCTCGATTTTCACCGCGAGCAGCCAGATGTTCCGCAACACGCATCTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGC  >  NC_000913/1781464‑1781895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑