Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,881	C→T	A15T (GCG→ACG)	ddlA ←	D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,881	0	C	T	100.0%	18.6 / NA	9	A15T (GCG→ACG)	ddlA	D‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

ATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACGGGAAGCTAATTTATTCC  >  NC_000913/400756‑401111                                                                                                                              |                                                                                                                                                                                                                                       aTTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGAttgttg                                                                                                           <  1:117392/251‑1 (MQ=255)                  cGTGCCCTTGCGCTTGTTTATCAATGCCCCGCAGCACAACGTCGACGCCACCTTCCTCATTGGCATCCACAATGTTTTATGCCCATTGTAGAGACAATTCCTGCTCCGTTGATTTTCCACCAAAAACAATTCCTACGCGCCGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGAGCATACATTACCACGACCAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTACTGACa                                                                                           >  1:21771/1‑250 (MQ=255)                          tGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCGGCTGACAAAGCGTGc                                                                                   <  1:59832/250‑1 (MQ=255)                                       cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGCTTGCAGAGACACTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTcc                                                                                                                                                                                                >  1:115145/1‑128 (MQ=255)                                       cAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAANTGCNTCGANAANGTTTTTTGCCGATTGNAGAGACACTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCNNCNNNCNGTTTTTcc                                                                                                                                                                                                <  2:115145/128‑1 (MQ=255)                                                                 cGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTAATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTgc                                           <  1:152578/251‑1 (MQ=255)                                                                               ggCATCGACGATGTTTTTTGCGGATGGCAGAGACCCTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTGTTCCATCTTAAAAACCTATCCCGGCGAACACAAAGTGCATACATCATGACGACGAAACGGGGGATGCGCGGCCTTCTGAAAGTTTGTTGCGATGGGCTGTTGGGAAAGCGGGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGAtgtg                                            <  1:76284/236‑1 (MQ=255)                                                                                               tttGCCGATTGCAGAGACACTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACTTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACGGGacg              <  1:159070/250‑3 (MQ=255)                                                                                                                 acTTCATGTTCCGTTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTTCATCTTAAAAACATATCCCGTCTAACACAAAGTGCATACATTACCACGACACAACGGGGGATTCGCGGCCTTCTGACAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACGGGAAGCTAATTTATTcc  >  1:86073/1‑244 (MQ=255)                                                                                                                              |                                                                                                                                                                                                                                       ATTGCTGGCATCGCTGACGTGCCATTGCCCTTGTTTATCAATGCCCAGCAGCACAACGTCGAAGCGACTTTTATCAATGGCATCGACAATGTTTTTTGCCGATTGCAGAGACACTTCATGTTCCGCTGATTTACCACCAAAAACGATTCCTACCCGCAGTTTTTCCATCTTAAAAACCTATCCCGTCTAACACAAAGTGCATACATTACCACGACAAAACGGGGGATTCGCGGCCTTCTGAAAGATTGTTGCAATCTTCTGCTGACAAAGCGTGCAACGTACTGGTGAAGAAAGTGCGTTATCTCAAAGATGTGCGCAAGATCACAAAAATGATGAACGGGAAGCTAATTTATTCC  >  NC_000913/400756‑401111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑