Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,549	C→T	I435I (ATC→ATT)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,549	0	C	T	100.0%	21.7 / NA	10	I435I (ATC→ATT)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

GCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTC  >  NC_000913/4182328‑4182762                                                                                                                                                                                                                              |                                                                                                                                                                                                                      gcACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGCGACACAGCTGAAAGCCTGTTAGCGAAACTGTTCTTCTCCGAAGACCGTTATGACATGTCTGCGGTTGCTCGTATGAAGTTTAACCGTTATCTGCTGCGCGAAGAAATCGAAGATTCCGGAATCCTGAGCAAAGACGACATCCTTGATGTTATGAACAAGCTCATCGCTATTCGTAACGGTAAAGGCGAAGTCGATGatat                                                                                                                                                                                          >  1:122034/1‑251 (MQ=255)                 aCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCCGCTGAAAGCATGTTCGAGAAACTGTTCTTCTCCGAAGCCCGTTATGACTTGTCTGCGGTTGATCGAATGAAGTTCAACCGTTCTATGCTGCGCGACGAAATCGCAGGTTCCGGTATCCTGAGCAAAGACGACCTCATTGATGTTATGAAAACGCTCATCGATATTCGTAACGGTAAAGGCGCAGTCGCTAATATCGACCACCTCGGCAAc                                                                                                                                                                          >  1:164479/1‑251 (MQ=255)                                                 ggTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATTCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCgg                                                                                                                                           >  1:59206/2‑250 (MQ=255)                                                                                 gTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATTCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTTGCCTGGTGCGTGTag                                                                                                          <  1:46600/251‑1 (MQ=255)                                                                                              gACCGTTATGACTTGTCTGCGGTGTGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACTACATCAGTGATGTTATGAAAAAGCTCATCGATATTCGTAACGGTAAAGGCGAAGTCGATGATATCGACCTCCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGCAATCCCGTTCCGCTTTGGCCTGGTACGTGTAGAGCGTGCGGTGaa                                                                                             <  1:72967/251‑1 (MQ=255)                                                                                                                       gTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAATGTGCCGGTATCCTGAGCAAAGACGACATCATTTATGTTATGAAAAACCTCATCGATATTCGTAACGGTAAAGGCGAAGTCGAGGATATCGACCACCTCGGc                                                                                                                                                                             <  1:36837/146‑1 (MQ=255)                                                                                                                       gTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAACAGCTCATCGATATTCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTg                                                                    >  1:39186/1‑251 (MQ=255)                                                                                                                             tGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGGATCCTTAGCAAAGACGACAGCATTGATGTTATGAAAAAGCGCATCGATATTCGTAACGGTAAAGGCGAAGTTGATGATATCGACCGCCTGGGTAACCTTCGTATCTGTGCCGGTGGTGAAGTGGCGGGATATCAGGTGCTAGTGGGCGTGGTACGTGTAGAGCTTTCGCTGAAAGAGCGTCTGCCTCTGGGCGATCTGGATAc                                                               <  1:64719/250‑1 (MQ=255)                                                                                                                                 gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTTCGGTATCCTGAGCAAAGACTACATCATTGGTGGTATGAAAAAGCTCATCGATATTCGTAACGGTCAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGACAACAAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGAGCGGTGAAAGAGCGTCTGTCTCTGAGCGATCTGGATACCCTg                                                           >  1:26200/1‑250 (MQ=255)                                                                                                                                                                                         acgacATCATTGATGTTATGAAAAAGCTCATCGATATTCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATTCCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGttcttc  <  1:3854/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                      GCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTC  >  NC_000913/4182328‑4182762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑