Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,215,552 C→T T229T (ACC→ACT)  mlrA → DNA‑binding transcriptional activator MlrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,215,5520CT100.0% 11.7 / NA 6T229T (ACC→ACT) mlrADNA‑binding transcriptional activator MlrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/4);  total (2/4)

GCACGTAAAAAACAGGGTAAAGATGCGCTGGTGGTTGGCTGGAATATTCAGGATACCACCCGTCTGTGGCTGGAGGGCTGGATTGCCAGTCAACAAGGATGGCGCATTGATGTCCTCGCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAG  >  NC_000913/2215334‑2215789
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             
gCACGTAAAAAACAGGGTAAAGCTGCGCTGGTGGTTGGCTGGAATATTCAGGATACCACCCGTCTGTGGCTGGAGGGCTGGATTGCCAGTCAACAAGGATGGCGCATTGATGTCCTCGCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACTAGTTGGCAAGAACAAGGCCATGATATTTTccc                                                                                                                                                                                                               >  1:35955/1‑251 (MQ=255)
                                                                                                                                              cTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACATCCGCCCAACAGCAGCAACTCACTAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTCTATGGCGTTAGGCGGAATTGGTGGCGTc                                                                  >  1:94769/1‑250 (MQ=255)
                                                                                                                                                gAACTATTCGAAGGCCGTACATTGCTGGTGTGTTGCGGTTAAAATCGAACCTCCGCCCAACAGCAGCAACTCACTAGTTGGCAAGAAGAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATTTGCTTTACTGTACAATCCTTTCGTTAACATAATGAGTGCATTGTGCGCATAGGTAGAATTTGGGTCATCGCCCTTTGCCTGTTTATGGCGTTAGGCGTAATTGGTGGCGTCAt                                                                <  1:156814/250‑1 (MQ=255)
                                                                                                                                                                          ggtgtggtgCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACTAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGc                                      <  1:12258/250‑1 (MQ=255)
                                                                                                                                                                                     gTGAAAATCGAACCTCTGCCCAACAGCAGCAACTCACTAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTCTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTAttt                                         <  1:87244/236‑1 (MQ=255)
                                                                                                                                                                                                             agcagcAACTCACTAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAATGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCGTGTTCCTGTGTATGGCGTTAGGCGGAATGGGTGGCGTCATGCTCTCAGGGTATGCCTTTAGTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAg  <  1:81947/251‑1 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             
GCACGTAAAAAACAGGGTAAAGATGCGCTGGTGGTTGGCTGGAATATTCAGGATACCACCCGTCTGTGGCTGGAGGGCTGGATTGCCAGTCAACAAGGATGGCGCATTGATGTCCTCGCCCACTCGCTCAATCAACTACGCCCTGAACTATTCGAAGGCCGTACATTGCTGGTGTGGTGCGGTGAAAATCGAACCTCCGCCCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAG  >  NC_000913/2215334‑2215789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑