Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	438,773	C→T	D469N (GAT→AAT)	dxs ←	1‑deoxy‑D‑xylulose‑5‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	438,773	0	C	T	100.0%	23.2 / NA	10	D469N (GAT→AAT)	dxs	1‑deoxy‑D‑xylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

GCTGGCGGCCATTTCCAGAATTAACGCTTCATCAAGCGGTTTCACAAAACGCATATCGACCAGCGTGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCG  >  NC_000913/438531‑439011                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               gctggcGGCCATTTCCAGAATTAACGCTTCATCAAGCGGTTTCACAAAACGCATTTCGACCAGCGTGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATTGTTATAGt                                                                                                                                                                                                                                        <  1:3442/251‑1 (MQ=255)      cggcCATTTCCAGAATGAACGGTTCATCAAGGGGTTTCACAAAACGCATATCGACCAGCGTGGCGTTCAGCGATTCGGCGGCTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTGTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCGCGCCGACCGCGTTGCCACGCCGGTATCGCACCGCTGACGGGCCATTGTTATAGTGATAg                                                                                                                                                                                                                                   <  1:70331/251‑1 (MQ=255)        gcCATTTCCAGAATTAACGCTTCATCAAGCGGTTTCACAAAACGCATATCGACCAGCGTAGAGTTCAGCGATTCGGCGTCTTTCGCCGCTTCTGGCATCAGCGTAACAAAGATAAGGATCGCCAGTTTCTCGCCAAGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCTAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATTGTTATAGTGATAGcc                                                                                                                                                                                                                                 >  1:148265/1‑251 (MQ=255)                                       gTTTCACAAAACGCATATCGACCAGCGTGGCGTTCAGAGATTCGGCGACTTTCGACGATTCTGGCATCAGCGTAACAAAGATAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGGAGTTTTTCTAGCGGCGTCAGTTACACGCTGACCGCGTTGCCAGGCGGGGAGCGCACCGCTGACGGGCCATTGTTTTAGTGATAGCCGGTATCGCGCATCTGGGGACATTCGTTTTc                                                                                                                                                                                                   >  1:76132/1‑250 (MQ=255)                                                                                 cGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATTGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCTGTATGCAGCGCAgn                                                                                                                                                        <  1:136085/251‑2 (MQ=255)                                                                                                                            gATCGCCAGATTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATTGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCAc                                                                                                             >  1:46457/1‑251 (MQ=255)                                                                                                                                               cgacgCTTCACAATGCCTTTGCCAATTTGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATTGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGtn                                                                                          <  1:29087/251‑2 (MQ=255)                                                                                                                                                                          ggTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATTGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGt                                                                                                                                                                                       <  1:117290/131‑1 (MQ=255)                                                                                                                                                                          ggTAGTTTNTNNNGNNGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCNCTGACGGGCCATTGTNATNGTGANAGCNGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGt                                                                                                                                                                                       >  2:117290/1‑131 (MQ=255)                                                                                                                                                                                                                                       cTGACGGGCCATTGTTATAGTGATAGCCGGTATAGAGCCTCTGGCGACATTCGTTTTCATCGCTCGGGGTCATACTGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCACAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGCCCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGAAGTAAATcgcg  >  1:82248/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               GCTGGCGGCCATTTCCAGAATTAACGCTTCATCAAGCGGTTTCACAAAACGCATATCGACCAGCGTGGCGTTCAGCGATTCGGCGACTTTCGCCGCTTCTGGCATCAGCGTACCAAAGTTAAGGATCGCCAGTTTCTCGCCACGACGCTTCACAATGCCTTTGCCAATTGGTAGTTTTTCCAGCGGCGTCAGTTCCACGCCGACCGCGTTGCCACGCGGGTAGCGCACCGCTGACGGGCCATCGTTATAGTGATAGCCGGTATAGAGCATCTGGCGACATTCGTTTTCATCGCTCGGGGTCATAATGACCATTTCCGGTATGCAGCGCAGGTAAGAGAGATCAAAAGCACCCTGATGGGTTTGACCGTCAGCACCAACAATGCCCGCGCGGTCGATGGCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCG  >  NC_000913/438531‑439011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑