Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,824,748	C→T	T78T (ACC→ACT)	trmH →	tRNA (Gm18) 2'‑O‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,748	0	C	T	100.0%	23.5 / NA	11	T78T (ACC→ACT)	trmH	tRNA (Gm18) 2'‑O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

GAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCC  >  NC_000913/3824501‑3824980                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         gaaaTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAACAGCTGGGGACAGGGGAAAACACACCGCACTAtt                                                                                                                                                                                                                                       >  1:8987/1‑251 (MQ=255)                                                  gCTCGCCAGGCTGCAGCCTGATCTGACCGTTTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGATCCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGTTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCTCTCTATTGGCGATGCCGTCGCTCATCTCAAATGCCAGGGCATGCAGATTCTGGCa                                                                                                                                                                                       <  1:21948/250‑1 (MQ=255)                                                                   cTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGACGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCCTGGCTTCGGCCGCGGCGGGGAGTCACCGCTTGGTACAGGTGAAAACACACCCCCCTATTGGCGATGCCGTCGCCCCTCTCACAGGCCCGGGCCTGCCGATTCTGGCAACCCCCCTTTCTGTTAAc                                                                                                                                                                     >  1:86691/1‑251 (MQ=255)                                                                                                                     gCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACTATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAttttn                                                                                                                     <  1:104598/249‑2 (MQ=255)                                                                                                                          tattCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACTATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTg                                                                                                                            <  1:11109/237‑1 (MQ=255)                                                                                                                              cGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACTATTTGCGATGCCGTCGCTCATCTCAAAGGCCCGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTTATTACACTCGCCCGACCTGCATTTTTATGGGACAggg                                                                                                          >  1:143507/1‑250 (MQ=255)                                                                                                                              cGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGGGAAAACACACCGCACTATTGGCGATGCCGTCGCCCCTCTCAAAGGCCAAGGCATGCAGATTCTGGCAACCCATCTTTCTGCTAACGCTGTCGATTTTCGCGAAATTTATTACACTCGCCCGACCTGCATTTTGATGGGACAggg                                                                                                          >  1:143500/1‑250 (MQ=255)                                                                                                                                                 gCGTACATGAAGTGCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACATCTGGGTACAGGTGAAAACACACCGCACTAGTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGAGTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTccn                                                                                                                                                       <  1:28994/187‑2 (MQ=255)                                                                                                                                                                               gTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACTATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCan                                                                                           <  1:100680/217‑2 (MQ=255)                                                                                                                                                                                 aGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACTATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTGCCGCGAAATTGATTACACGCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGTCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGCcatca                                                        <  1:50094/250‑1 (MQ=255)                                                                                                                                                                                                                                         gAAAACACACCGCCCTATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGccc  >  1:52829/1‑248 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         GAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCC  >  NC_000913/3824501‑3824980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑