Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,439	G→A	A467T (GCG→ACG)	dgcJ →	diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,439	0	G	A	100.0%	15.5 / NA	7	A467T (GCG→ACG)	dgcJ	diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

GCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCC  >  NC_000913/1873241‑1873661                                                                                                                                                                                                       |                                                                                                                                                                                                                               gCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCCTCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTCTTTACACGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCg                                                                                                                                                                            >  1:157358/1‑251 (MQ=255)                                                                         gATGAATTNTNNNTNNTTCTGGTCGATTCGACGCCGCAAGTTGCAGAACAACTGCCTGAANGAATCGAAAAACGTCNGCNACATNTCGNGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACACGATGAAAGAAAACGATACGTTACATTATTCGTATAAAGCTTCCGATGAGCGTATATATGACAATCANCAGNACagcnnanaaaagcnnn                                                                                                                                      >  2:120744/1‑202 (MQ=255)                                                                          aTGAATTCTGCATCATTCTTGTCGATTCAACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTTCAACATATCACGCCGCAGAAAGAGATCGTCTTCTCTTCCGGTATGGACACGATTAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTcc                                                                                                                                                                                    <  1:106676/170‑1 (MQ=255)                                                                                      tcatTCTTGTCGAGTCTACGCCGCAAATTGCAGCGCAACTGCCTCAACGTATTTAAAAACTTCTGCAACATATCGCGCTGCAGAAAGATATCTTCTTCTCTGCCGTTGGTTACACGATGAATGAAAACGAGACTTTACATGATGCTTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAg                                                                                                                                                            <  1:172572/182‑1 (MQ=255)                                                                                                                    cagcaCAACTGCCTGAACGTATCGAAAAACGTCTGCAACCTATCGCGCCGCAGAAAGCGCTCGGCTTCTCTTCCGGTATTTACACGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCAt                                                         >  1:91191/1‑251 (MQ=255)                                                                                                                                                                   ccgcAGAAAGAGATCGGCTTCTCTTCTGGTATTTACACCATGAAAGAAAACGATGCGTCACCTGATGCGTATATCGCTTCCGATGAGCGATTCTATCTGAATCAGCAGAATATAAACAGCCGTTCACGATCACCTGTGGTGGTTGTTTGCTTGTAATCTCAGGAGTGTAAACTGACTGTAATGGCTCACGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCCCACGAAGGTACTGACTTCcgcgg          >  1:162677/1‑250 (MQ=255)                                                                                                                                                                           agagATCGGCTTCTCTTCCGGTATTTACACGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATTATATCCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTAccc  <  1:118063/251‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                               GCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTCGATTCGACGCCGCAAATTGCAGCACAACTGCCTGAACGTATCGAAAAACGTCTGCAACATATCGCGCCGCAGAAAGAGATCGGCTTCTCTTCCGGTATTTACGCGATGAAAGAAAACGATACGTTACATGATGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCC  >  NC_000913/1873241‑1873661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑