Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,159,473	C→T	A363V (GCC→GTC)	mdtC →	multidrug efflux pump RND permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,159,473	0	C	T	100.0%	26.4 / NA	12	A363V (GCC→GTC)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

ACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGA  >  NC_000913/2159231‑2159674                                                                                                                                                                                                                                                   |                                                                                                                                                                                                          aCTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCGCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGTCGTTTCg                                                                                                                                                                                                    <  1:154207/250‑1 (MQ=255)                                        gACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATg                                                                                                                                                                <  1:70196/247‑1 (MQ=255)                                                                      ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATg                                                                                                                                                                <  1:132586/217‑1 (MQ=255)                                                                                         ccGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTCTCTCGGTGGCGCTGGTGATTCTGGTGGGGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTCTTCCCGTCGTTTCGGTGCCGGTTTc                                                                                                                                                                                         >  1:106407/1‑173 (MQ=255)                                                                                                                      ggATCGCTCCCCCACCATTCGCGCCGCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCGGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTAGTATTCCCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTTCCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATg                                                                                                                         <  1:144784/208‑1 (MQ=255)                                                                                                                       gATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGCGGCGCTGGTGATTCTGGTGGTGTTTTTATTCATGCGCTCGGGTCGCGCCACTATTATTCCCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGCTTCAGTCTCAATCACCTTTCGTTAATGGCGCTCACAATCGCTACTGGTTTCGTGGTGGATGACGCCATCgg                                                                             >  1:123509/1‑250 (MQ=255)                                                                                                                              cccccACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCCCTAGTATTCCCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCGTCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGc                                                                        <  1:86833/249‑1 (MQ=255)                                                                                                                                                           gTCGAGCAAACGCTGATTTTCCCTGTGGCGCCGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCCGGTCGCGCCACTACCATTCCCGTCGTTGCGGTGCCGGATTCGCTGATTGCTACGTTTTCCGCGATGTACCTGTGCGGCTTCAGTCTCAATAACCTTTCCTGAACGTCGCTCTTCACCGCTACTGGCTCCGTGGTGGATGCCGCCATCGTGGTGCTTGAAAACATTGCACGTTATCTGGAAGCggg                                         <  1:144995/251‑1 (MQ=255)                                                                                                                                                                             aTCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCGTTCGTTAATAGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAg                        <  1:87805/250‑1 (MQ=255)                                                                                                                                                                                       cgcTGGAGATTCTGGTGGTGTTTTTGTTCCTGCGCTCGGGTCGCGCCACTTTTATTCAAGTCGATTAGGTGTCGGCTTCGCTGATCGGTACGTTTGCCGCGATGTCCCTGTCCGGATTCAGTCTCACTAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGCAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCGGAACTGCa              >  1:152090/1‑250 (MQ=255)                                                                                                                                                                                            gTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTACCGTCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCa              >  1:171677/1‑245 (MQ=255)                                                                                                                                                                                                                                   gcCACNNTTNNNCCNNTCGTTTCGGTGCCGGTTTCGCTGNTTGGTACGTTTGCGGGGATGTACCTGTGCNGNTNCAGTNNCAANAACCTTTAGTNAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTNGNGATGGAAAACATTGCACGTCATCTGGAAGCGNGAATGAAACCGTTGCAAGCCGCACNGCAANNTANtnnnnn  >  2:123094/1‑213 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                          ACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGA  >  NC_000913/2159231‑2159674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑