Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,152,036	G→A	P355S (CCG→TCG)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,152,036	0	G	A	100.0%	23.8 / NA	10	P355S (CCG→TCG)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

TTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGGTTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATAGCGATACGGTTCTGCGGCACCTTCTTCGCCAACCAGCGCCAGCAGTTCAGGGGTCGCTTCAACCATCGACAGTTCAGAAACCAGCACCTGAATATCTTTCAGGAAC  >  NC_000913/4151842‑4152265                                                                                                                                                                                                   |                                                                                                                                                                                                                                      ttCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAGACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCg                                                                                                                                                                                <  1:168350/250‑1 (MQ=255)                               cAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCAGGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATGCCCACAGGTCTTCGTTTTGTGGCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCtt                                                                                                                                                                            <  1:69318/224‑1 (MQ=255)                                                 ggTATGACGCGTGCTCGCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTGGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGaan                                                                                                                               <  1:135152/251‑2 (MQ=255)                                                                  ccTGACGGAGAGCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGAGCGCCGTTGTCAATAATGCCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGtt                                                                                                                                                                                                                            <  1:173448/141‑1 (MQ=255)                                                                       cGGATATCACTACGGACAAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATAGCg                                                                                                         >  1:130238/1‑251 (MQ=255)                                                                                    ggACCAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGATGGCGATAATCCCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCATTATGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATAGCGATACGGTTCTGCg                                                                                            >  1:87200/1‑251 (MQ=255)                                                                                                cgccgcAACATTTCACGCGGAGCAGGGTGTCGAGCAGATCGCCGTTGGGGATACTGCGCATGCCACACGCCTGAAGTGACTGGTAGCCGGCGTAGAGCGATTCGCACCGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTATTGAAGTTCTTCg                                                                                                                                                                                >  1:136598/1‑155 (MQ=255)                                                                                                cGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATAGCGATACGGTTCTGCGGCACCttcttcn                                                                                <  1:40862/251‑2 (MQ=255)                                                                                                             naCGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATAGCGATACGGTTCTGCGGCACCTTCTTCGCCAACCAGCGcc                                                                    >  1:99010/2‑250 (MQ=255)                                                                                                                                                                              aCTGGTAGCAAGCGTAGAGCGATTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCAGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATATCGATACGGTTCTGCGGCACCTTCTTCGCCAACCAGCGCCAGCAGTTCAGGGGTCGCTTCAACCAGCGACAGTTCAGAAACCAGCACCTGaaagatattcaggtgc  <  1:137305/251‑15 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                                                                                                      TTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCGCAGGGTGTCGAGCAGATCGCCGTTGGCGATAATACCCATGCCACACGCCTGAAGTGACTGGTAGCAAGCGTAGAGCGGTTCCCACAGTTCTTCGTTTTGTGTCAGCAGGCCTTCTGGTTTTGGCAGTTCTTCGCCTTTCAGGCGCGCTTCCAGCCATGCCTGTGTCGCCATCAGGCGAGAACGCAGGTTTTTCATCAGATAGCGATACGGTTCTGCGGCACCTTCTTCGCCAACCAGCGCCAGCAGTTCAGGGGTCGCTTCAACCATCGACAGTTCAGAAACCAGCACCTGAATATCTTTCAGGAAC  >  NC_000913/4151842‑4152265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑