Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,512,917	C→T	G165S (GGC→AGC)	fecC ←	ferric citrate ABC transporter membrane subunit FecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,512,917	0	C	T	100.0%	15.3 / NA	7	G165S (GGC→AGC)	fecC	ferric citrate ABC transporter membrane subunit FecC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

CAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGT  >  NC_000913/4512690‑4513150                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          cAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCTCATACAAAAGGCCGACAGcgcg                                                                                                                                                                                                                     >  1:137247/1‑250 (MQ=255)                cAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCCGGTTCAGTTGCTTCGCCAGCAGCCACACCACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAACGATGCCGTAAGCCTGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCTCATACCAAAGGCCGACAGCGCGATACCCGCGCGGATc                                                                                                                                                                                                      >  1:124513/1‑250 (MQ=255)                                                                                                                                                   ngTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCTCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGt                                                                                                                                                          >  1:32355/2‑163 (MQ=255)                                                                                                                                                         acacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCTCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAAGCCGCCGCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCAGGCCGCGATGAAGGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAgc                                                            <  1:158178/251‑1 (MQ=255)                                                                                                                                                          cacTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCTCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAgcgcg                                                                     >  1:7137/1‑241 (MQ=255)                                                                                                                                                                                                         ccAGCAGCAGGGTGATGCGGGTAAGGCTCATACAACAGGCCGACAGCGCGCTACCCGCGAGGATCAGTTTGTTTATGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTAAATGCCGAGCAGTGAAGgag              >  1:66034/1‑249 (MQ=255)                                                                                                                                                                                                                   ngTGATGCGGGTAAGGCTCATACAACAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGCTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGt  >  1:135499/2‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                          CAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGACAGCGCGATACCCGCGAGGATCAGTTTGTTTCTGTCATGGGTATGACGAAATCCGCCTCCTGCGGTCATGACCAGCAGCCAGCTCACGCCGCCCCCGCATGCCGCGATGAATGACAGAGAATAGCCTGCAATCGGCGTCGGACTCAGCGCGCTGGTAAGCGCCATAGCCAGCGCCGCGCCGCTGTTAATGCCGAGCAGTGAAGGAGAGGCCATTGGGT  >  NC_000913/4512690‑4513150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑