Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,990,161	C→T	L161L (CTG→TTG)	tyrP →	tyrosine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,990,161	0	C	T	100.0%	19.6 / NA	8	L161L (CTG→TTG)	tyrP	tyrosine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

TCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTCTGCAATCCCGGTGATTTTTACGTCGTTTGGTTTTCACGGTAGCGTGCCGAGTATTGTCAGCTATATGGATGGCAACATTCGTAAGCTACGCTGGGTGTTTATAATCGGTAGTGCGAT  >  NC_000913/1989946‑1990351                                                                                                                                                                                                                        |                                                                                                                                                                                               tCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGCGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGCATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAATTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGTTGGTACTACTGCTGCCGCATATTCACAAAGTGAAt                                                                                                                                                             >  1:67225/1‑251 (MQ=255)                                                                         ggACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGTTGGtacn                                                                                                                                                                                         <  1:104690/151‑2 (MQ=255)                                                                                         gTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGGCGGTGGCGTCGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAAACGTTTTCTGTTCAGCGCCAAGATTATTTTTTTGGTGGTAATGTTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTCTGCAATCCCGGTGGTTTTTAc                                                                                                  >  1:80498/1‑222 (MQ=255)                                                                                                       gCGTGCTGTTGTTCACTTTTGTGGCCGGTGGCGTGGTTTGTGTCGGANCTTCACTGGNNGANTTATTNANNNGNNNTCNGTGCAGCGCCAAGATTATTNTTNNGGTGGTNATGTTGGTACTACTGCTGCCGCatat                                                                                                                                                                          <  2:35873/136‑1 (MQ=255)                                                                                                       gCGTGCTGTTGTTCACCTTTGTTGCCGGGGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGTTGGTACTACTGCTGCCGCatat                                                                                                                                                                          >  1:35873/1‑136 (MQ=255)                                                                                                                 gttCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGTTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTCTGCAATCCCGGTGAGTTTTACGTCGTTTGGTTTTCACGGTAGCGTGCCGAGTATTGTCAGCTATATGGATGGc                                              <  1:150349/250‑1 (MQ=255)                                                                                                                                                  cGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGTTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTCTGCAATCCCGGTGATTTTTACGTCGTTTGGTTTTCACGGTAGCGTGCCGAGTATTGTCAGCTATATGGATGGCAACATTCGTAAGCTACGCTGGGTGTTTATAATcn            <  1:20268/251‑2 (MQ=255)                                                                                                                                                                              cGTTTTCTGTTCAGCGACAAGATTATTTTTCTGGTGGTAATGTTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTCTGCAATCCCGGGGATTTTTACGTCGTTTGGTTTTCACGGTAGCGTGCCGAGTATTGTCAGCTATATGAATGGCAACATTCGTAAGCTACGCTGGGGGTTTATAATCGGTAGTGCGAt  >  1:52778/1‑233 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                               TCTTAATGTATGCTCTGACTGCGGCATACATCAGCGGTGCCGGTGAATTGTTGGCCTCCAGCATCAGCGACTGGACAGGTATTTCTATGTCGGCAACCGCTGGCGTGCTGTTGTTCACTTTTGTTGCCGGTGGCGTGGTTTGTGTCGGAACTTCACTGGTCGATTTATTTAACCGTTTTCTGTTCAGCGCCAAGATTATTTTTCTGGTGGTAATGCTGGTACTACTGCTGCCGCATATTCACAAAGTGAATCTTTTAACCCTGCCGTTGCAACAGGGGCTGGCTCTGTCTGCAATCCCGGTGATTTTTACGTCGTTTGGTTTTCACGGTAGCGTGCCGAGTATTGTCAGCTATATGGATGGCAACATTCGTAAGCTACGCTGGGTGTTTATAATCGGTAGTGCGAT  >  NC_000913/1989946‑1990351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑