Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	200,942	C→T	A154V (GCC→GTC)	skp →	periplasmic chaperone Skp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	200,942	0	C	T	100.0%	26.5 / NA	11	A154V (GCC→GTC)	skp	periplasmic chaperone Skp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

GCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACTTTTGCTCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGAC  >  NC_000913/200714‑201162                                                                                                                                                                                                                                     |                                                                                                                                                                                                                             ncGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCAAGACTTTTGCTCAGAAAGCGAAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTaa                                                                                                                                                                                                         >  1:29605/2‑250 (MQ=255)            aaGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACTTTTGCTCAGAAAGCGCAGGCTTTTGAGCAGGATCTCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTGCTGAAACAGGTTAAATAAGTAATGc                                                                                                                                                                                              <  1:116886/250‑1 (MQ=255)                                                tttGCTCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTg                                                                                                                                                           <  1:149584/249‑1 (MQ=255)                                                                             ggATCGCGCAAGTCGTTCCAACGAAGACCGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCACCAGCCCGGCTATCGATCTGGTTGTTTATGCAAACGCCGTTGCTTACAACAGCAGCGCTGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCg                                                                                                                            >  1:149566/1‑251 (MQ=255)                                                                                  gcgcACGTAGTTCCAACGAAGAACGCGGCAAACTGGTTACCTGTATCCAGACTGCTGCGAAATCAGTTGCCAACAGTCAGGATATCGATCTTGTTGTTGATGCAAACCACGTTGCTTACAACAACAGCGATGTAATAGACCTCACTGTTGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCCATTCGACTGGCTGATTTAGCGCAACAGTTGGATGCAGAACTACACGGCGATGGCGATATCGtcatc                                                                                                                       >  1:171043/1‑251 (MQ=255)                                                                                                           cgGCAAACTTGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAGCTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGGCCATGCAATCTg                                                                                              <  1:97060/251‑1 (MQ=255)                                                                                                                         cTCGTNNCCNNNCTNNTGTGAAATCCGTTGCCAACAGCCNGGATATCGATCTGGTTGTTGATGCAAACGNCNTNGCTTNNAACNGCAGCGCTGTNAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGTAATGCCTNCNATTCGACTGGCTGATTTAGCGCAGCCGTTGGNTGCAGAACTACACGGTGCTGGCGANATCGnnatna                                                                                                                      >  2:127504/1‑213 (MQ=255)                                                                                                                         cTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTTCTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGGAATGCCTGCAAGTCGACTGGCTGAGTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCAc                                                                                                                     <  1:127504/214‑1 (MQ=255)                                                                                                                                                                                       gCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAAGTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAGTACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCggn                  <  1:29554/251‑2 (MQ=255)                                                                                                                                                                                                    cTTACAACAGCAGCGATGTAAAAGACATCACTGTCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCACTCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGAccc       >  1:69583/1‑249 (MQ=255)                                                                                                                                                                                                       acaacaGCAGCGATGTAAACGACATCACTGTCGACGTACTGCCACAGGCTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTCCACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCATTCTGCACAAACAGGTCACCTTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAggcc  >  1:52582/1‑249 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                             GCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACTTTTGCTCAGAAAGCGCAGGCTTTTGAGCAGGATCGCGCACGTCGTTCCAACGAAGAACGCGGCAAACTGGTTACTCGTATCCAGACTGCTGTGAAATCCGTTGCCAACAGCCAGGATATCGATCTGGTTGTTGATGCAAACGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTACTGAAACAGGTTAAATAAGTAATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTCATGACCCAGGAC  >  NC_000913/200714‑201162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑