Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	273,075	C→T	L77F (CTC→TTC)	ykfC →	PF00078 domain‑containing protein YkfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	273,075	0	C	T	100.0%	21.6 / NA	9	L77F (CTC→TTC)	ykfC	PF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

CGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGGTGAAATTACAGCTCACAGACTGTGGT  >  NC_000913/272833‑273311                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             cGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAggn                                                                                                                                                                                                                                      <  1:135554/251‑2 (MQ=255)                    aaTCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAgg                                                                                                                                                                                                                                       <  1:106467/231‑1 (MQ=255)                                                                                              gTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTGTCATCAACGGGGGCCAATACCCCCGGCGCTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGCTGAGCTGCAAATCCTCAGGGATGAATTCTTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTATATCCCTAAAAGCAACGGCCAACTGCGACCCCTGGGTATCCCCGCGTTGCGGGATCGTAtt                                                                                                                                         >  1:155840/1‑251 (MQ=255)                                                                                               tCTGATAACACAACCAGAATGGCTGACTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGAGGGCGTGAACAAAACAATGCTACAGGCGAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAGGCCACTACCAGCCCTTGcc                                                                                                                                                                                                                     <  1:169831/174‑1 (MQ=255)                                                                                                                                                 nnnnnnaGNNNNNCCANACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTaaaa                                                                                                                                                                                           <  2:102715/144‑1 (MQ=255)                                                                                                                                                             cccATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGGGATTTTCATACGCtctc                                                                           >  1:57253/1‑250 (MQ=255)                                                                                                                                                                                         caaaacaaTGCTCCAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTcc                                              <  1:137280/251‑1 (MQ=255)                                                                                                                                                                                                      cAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTATTCTCAGGACACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTc                                                                                                                                     >  1:162467/1‑151 (MQ=255)                                                                                                                                                                                                                                      cAGGGATGAATTATTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGTGGAAATTACAGCTCACATACTgtggn  <  1:135800/250‑2 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             CGAGGTGAAGACCTTTGATAATCAGCGAAATGCAACGCAAGCTTGCCACATGGGCAGCCACCGATCCGTCCCTACGGATTCAACGGCTGCTGCGTCTGATAACACAACCAGAATGGCTGGCTGAAGCGGCGCGGATCACGCTTTCATCAAAGGGGGCCCATACCCCCGGCGTTGATGGCGTGAACAAAACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGGTGAAATTACAGCTCACAGACTGTGGT  >  NC_000913/272833‑273311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑