Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	441,479	C→T	intergenic (‑136/‑70)	xseB ← / → thiI	exodeoxyribonuclease VII subunit XseB/tRNA uridine 4‑sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	441,479	0	C	T	100.0%	19.8 / NA	9	intergenic (‑136/‑70)	xseB/thiI	exodeoxyribonuclease VII subunit XseB/tRNA uridine 4‑sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/5);  total (4/5)

CGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAA  >  NC_000913/441232‑441719                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ngTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGTCGc                                                                                                                                                                                                                                               >  1:40919/2‑251 (MQ=255)                                   ttCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAg                                                                                                                                                                                                              <  1:3365/250‑1 (MQ=255)                                                                                                                              ntctcAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGAGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCtgggagctt                                                                                                                  >  1:99558/2‑244 (MQ=255)                                                                                                                                                                                                  ggTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTg                                              <  1:54663/251‑1 (MQ=255)                                                                                                                                                                                                     gATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTgtn                                            <  1:28969/250‑2 (MQ=255)                                                                                                                                                                                                        gtgCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTcc                                        <  1:7892/251‑1 (MQ=255)                                                                                                                                                                                                                             ntGGTATACTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATAAAAGTCGCTTAAGGCTTGCCAACGACCCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGa                     >  1:17785/2‑249 (MQ=255)                                                                                                                                                                                                                                  ataCTTCCGCGCCTGGATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTcg               <  1:141797/250‑1 (MQ=255)                                                                                                                                                                                                                                               ttgATGCAGTCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACAATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAAACACCATCAACAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGCACATTCGGAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGaa  >  1:164477/3‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGTAACGGACTCAGGGCAAATAGCAAATAACGCGCAATGGTAAGGTGATGTGCACAGCAAAGCGATGTTAGTGGTATACTTCCGCGCCTGGATGCAGCCGCAGGTGTGGGCTGCTGTATTTTTCCCTATACAAGTCGCTTAAGGCTTGCCAACGAACCATTGCCGCCATGAAGTTTATCATTAAATTGTTCCCGGAAATCACCATCAAAAGCCAATCTGTGCGCTTGCGCTTTATAAAAATCCTTACCGGGAACATTCGTAACGTTTTAAAGCACTATGATGAGACGCTCGCTGTCGTCCGCCACTGGGATAACATCGAAGTTCGCGCAAAAGATGAA  >  NC_000913/441232‑441719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑