Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,953,258	G→A	A259T (GCG→ACG)	ilvE →	branched‑chain‑amino‑acid aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,953,258	0	G	A	100.0%	15.2 / NA	7	A259T (GCG→ACG)	ilvE	branched‑chain‑amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

CGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCAC  >  NC_000913/3953012‑3953434                                                                                                                                                                                                                                                       |                                                                                                                                                                                 cgccACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTTGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCTTATGAAGTGTTTATGTCCGGTACgacggc                                                                                                                                                                              <  1:157727/251‑1 (MQ=255)                               gATGGGAACGGTTATATCTCTGAAGGCGCGGGCGAAAACCTGTTTGAAGTGAAAGAGGGTGTGCTGTGCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGGGAGCAGGTGCTGTCGCGCTAATCCCTGTACCTGGCGGAGGAAGTGTTTATGTCCGGTACGACGGCAGAAATCACGCCAGTGCGCAGCGTAGACgn                                                                                                                                                <  1:17932/251‑2 (MQ=255)                                                         ngcAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGACGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATACAGGTTGGCGAAGACCGTtgtg                                                                                                                      >  1:15363/2‑251 (MQ=255)                                                                                    aGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGACGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGTCCGGTGTGGCCCGGTTACCAAACGCATTCAGCAAg                                                                                           <  1:60969/251‑1 (MQ=255)                                                                                                               cACCTCCACCGCTCTGCCGTGGATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTTCGTGCGCGGGTGCTGTCGCGCGAATCCCTGTTCCTGGCCGATGAAGTCGTTATGGCCGGTGCGACGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGcta                                                                                                                                 <  1:86823/186‑3 (MQ=255)                                                                                                                                                    atcatcAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGAACGACGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGACCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATc                            >  1:707/1‑250 (MQ=255)                                                                                                                                                                   aaaGAGCTGGGAATTGAAGTACGGGAGCAGGTGCTGTCGCGCGAATCCCTGTACCGGGCGGATGAAGTGTTGATGTCCGGTACGACGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGACCGGTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCg                                                                                  <  1:108770/181‑1 (MQ=255)                                                                                                                                                                             gAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTAAGACGGCAGAAATCACGCCAGGGCGGAGCTTAGAGGGTAATCAGGTTGGCGAAGGCCGGGGTGGCCCGGTTACCAAACGCATTCAGCAAGCTTTCTTCGGCCTCTGCACTGTGGAAACCGAAGATAAAGTGGGCTGGTTAGATCAAGCTAAGCAAGAAATACAAAAAATGGGACGgcac  <  1:145213/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                 CGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCAC  >  NC_000913/3953012‑3953434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑