Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,314,350	G→A	G111S (GGT→AGT)	ompW →	outer membrane protein W

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,314,350	0	G	A	100.0%	25.6 / NA	10	G111S (GGT→AGT)	ompW	outer membrane protein W
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CCTTACGTTTACTTATATGGCGACCGACAACATTGGTGTGGAATTACTGGCAGCGACGCCGTTCCGCCATAAAATCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTGGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTT  >  NC_000913/1314202‑1314528                                                                                                                                                     |                                                                                                                                                                                   ccTTACGTTTACTTATATGGCGACCGACAACATTGGTGTGGAATTACTGGCAGCGACGCCGTTCCGCCATAAAATCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCttt                                                                              >  1:38275/1‑251 (MQ=255)                               caTTGGTGTGGAATTACTGGCAGCGACGCCGGTCCGCCATAAAATCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGATTCCTGGGGAg                                                <  1:63672/251‑1 (MQ=255)                                      gtgGAATTACTGGCAGCGAAGCCGTTCCGCCATAAACTCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGCTTCCTGGGGAGCTGCCgg                                         >  1:140954/1‑251 (MQ=255)                                                   cAGCGACGCCGTTCCGCCATAAAATCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGACAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGAATTAACTACACCACCTTCTTTGATAATGGCTTTAAAGATCCTGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGATTCATGGGGAGCTGCAGGGCAGGGGGGGGtt                            >  1:120480/1‑251 (MQ=255)                                                            cGTTCCGCCATAAAATCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTg                   <  1:73186/251‑1 (MQ=255)                                                                             ggCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACtggtt  >  1:110366/1‑251 (MQ=255)                                                                               cgcccGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCCGGTATTAAATac                                                                                                                                  >  1:163938/3‑121 (MQ=255)                                                                                 nccNGGGNANNGNNGATNTTGCAACCGTGCATCATCTGCNNCCAACACTGNGGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTTCTTCCTTACGTTGGGNCAGTTATTANNTANACCACNTNNNTNNNTANTGGAGTTAACGATCATGGCNAANNGGCAGGNCTTGCCGACCTCAGTCTGAAAGATTc                                                        <  2:38275/192‑1 (MQ=255)                                                                                                          cGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTc                                                                                                                                                                >  1:5377/1‑64 (MQ=255)                                                                                                          cGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTAGTGATGCCAGCAGCAAATTc                                                                                                                                                                <  2:5377/64‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                                                   CCTTACGTTTACTTATATGGCGACCGACAACATTGGTGTGGAATTACTGGCAGCGACGCCGTTCCGCCATAAAATCGGCACCCGGGCGACCGGCGATATTGCAACCGTTCATCATCTGCCACCAACACTGATGGCGCAGTGGTATTTTGGTGATGCCAGCAGCAAATTCCGTCCTTACGTTGGGGCAGGTATTAACTACACCACCTTCTTTGATAATGGATTTAACGATCATGGCAAAGAGGCAGGGCTTTCCGATCTCAGTCTGAAAGATTCCTGGGGAGCTGCCGGGCAGGTGGGGGTTGATTATCTGATTAACCGTGACTGGTT  >  NC_000913/1314202‑1314528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑