Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	440,384	C→T	G240S (GGT→AGT)	ispA ←	geranyl diphosphate/farnesyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	440,384	0	C	T	100.0%	16.6 / NA	7	G240S (GGT→AGT)	ispA	geranyl diphosphate/farnesyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

CTATTAATACTTATTGTTTATTTATTACGCTGGATGATGTAGTCCGCTAGCGCTTCCAGTGCCGAGGTATCGAGTGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCT  >  NC_000913/440185‑440629                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cTATTAATACTTATTGTTTATTTATTACGCTGGATGATGTAGTCCGCTAGAGCTTCCAGTGACGAGGGATCGAGTGACTGTTCAGCCAGTTGTTTCAGAGACTGACGGGCATCGTCGCTCAGATCCCGGGCTTTCCTCCGGGCTTGCTCAAGACCCAGAAGTGAAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACTCTGGCGt                                                                                                                                                                                                                                                >  1:152857/1‑207 (MQ=255)                           aCGCTGGATGATGTAGTCCGCTAGCGCTTCCAGTGCCGAGGTATCGAGTGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCAGCACTCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATg                                                                                                                                                                           <  1:67454/250‑1 (MQ=255)                                                                                                cAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACTCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGTCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                                                                                                      <  1:139567/250‑1 (MQ=255)                                                                                                      cTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACTCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                                                                                               >  1:64253/1‑251 (MQ=255)                                                                                                                     gATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACTCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTGAATGCACCAAGGCGAACGGCGGCGCGAATCAATg                                                                                <  1:59252/251‑1 (MQ=255)                                                                                                                               cGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGAAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACTCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATACTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGtttt                                                                      >  1:127361/1‑251 (MQ=255)                                                                                                                                                                                                   ngCACTCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTCATGCCt  >  1:105393/2‑251 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CTATTAATACTTATTGTTTATTTATTACGCTGGATGATGTAGTCCGCTAGCGCTTCCAGTGCCGAGGTATCGAGTGACTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAATACGCTCAAGCGCGTCCAGAGGTACGTGTTTGCCTTCCGCGTCTAAATCTAATGCCT  >  NC_000913/440185‑440629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑