Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,055	C→T	T50T (ACG→ACA)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,055	0	C	T	100.0%	14.5 / NA	7	T50T (ACG→ACA)	gatY	tagatose‑1,6‑bisphosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/1);  total (6/1)

CAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG  >  NC_000913/2176881‑2177223                                                                                                                                                                               |                                                                                                                                                                         cAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGTGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCCTTTCGAGATTGTGa                                                                                               >  1:118384/1‑250 (MQ=255)                                    cAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCCGTGGATGGTGATATTGCTTCGCCATAGAGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGTGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGc                                                                                                                                       >  1:106624/1‑175 (MQ=255)                                             ccTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCCACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGTGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAAccgccg                                                                 >  1:109858/1‑236 (MQ=255)                                                                                      tGAATTGCCAGCGGATGGTGATATTGCTTCGCCATCGCGCTGCCCAGCGCCAACAGATTTTCTGTACCAGAATGAGTAAATGTGACAGGTGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCCCCACTTGCATCGTTTc                                                                                                         >  1:23272/1‑155 (MQ=255)                                                                                             ccactGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCCGCATGAGTAAATGTGCCAGGTGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCCTCGTTTCGGGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCgtttg  >  1:173592/5‑251 (MQ=255)                                                                                             ccAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGTGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCCCCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCgtttg  >  1:173597/1‑251 (MQ=255)                                                                                                                                 ccAGCGCCAACAAATTTTCTGTACCAGCATTAGTAAATGTTCCAGGTGTTCCTGGGATGATGTCTGGCTCATGCATGTGGGCAGCGGTTTCTACCACCACTTGCATTGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCAt                                                                         <  1:110769/144‑1 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                         CAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCCAGGCGTTCCGGCGATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTG  >  NC_000913/2176881‑2177223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑