Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	326,430	C→T	G273D (GGC→GAC)	betA ←	choline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	326,430	0	C	T	100.0%	33.7 / NA	12	G273D (GGC→GAC)	betA	choline dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/2);  total (10/2)

CTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGCCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACG  >  NC_000913/326229‑326662                                                                                                                                                                                                          |                                                                                                                                                                                                                                         cTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGATCCCGAACTGAAGGGCAGGGTAGAGGAAAAACGGTACTTTGCAATCATGTTGAAGACAAAACTCAAGCTGATCCTGAAGATTTTCGACGAAGACGGGTAATGCATGCACCAGCGGAATATAAAACTACGCCAGCAGATAAGCGCTGTCGACGCCGGAGCGTTGAAGGATCTGCGGTGAGGCAATCGCGCCTGcac                                                                                                                                                                                          >  1:121513/1‑250 (MQ=255)             ccAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCActtc                                                                                                                                                                             >  1:53084/1‑251 (MQ=255)                   ccgccAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGACGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTttgtt                                                                                                                                                                       >  1:137133/1‑251 (MQ=255)                             gCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATCTTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTgcg                                                                                                                                                             >  1:89277/1‑251 (MQ=255)                                      cACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGGGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCgg                                                                                                                                                          >  1:706/1‑245 (MQ=255)                                                             cacTGCCGGGCCGGGTAGAGGCAGACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGCTGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGAGGGTTGGGATGGTGCTGTCGCCTTCCAGcc                                                                                                                              >  1:133004/1‑250 (MQ=255)                                                             cacTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGATGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGcc                                                                                                                              >  1:26626/1‑250 (MQ=255)                                                                          ggTAGAGGGAAACCAGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTgg                                                                                                                                                     >  1:112806/1‑214 (MQ=255)                                                                                                   cTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCCGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCCGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGAtgt                                                                                       >  1:86520/1‑251 (MQ=255)                                                                                                                       ccAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGGCGCCTTCCTGCCAGTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTg                                                                       <  1:125943/247‑1 (MQ=255)                                                                                                                                                    ncGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGc                                                                                                                                                                                                        >  1:34193/2‑89 (MQ=255)                                                                                                                                                                                        gCCAGCAGTTCAGCGTTGTCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGTGACg  <  1:64096/251‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                         CTGGCACCAACGCCAGTGCCGCCAAACAGCCACTCCGCACCGATTTTCGGCTGGTTCCACCACTGCAGGGCAGGGTAGAGGGAAACCGGTTCTTTGCACTCATATTGCAGATACATCTCCAGATGATCCTGAAGATTTTCGCCGACGCCGGGTAATTCATGCACCAGCGGAATATCAAACTCCGCCAGCAGTTCAGCGTTGCCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACG  >  NC_000913/326229‑326662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑