Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,050,689	C→T	G26E (GGA→GAA)	yihA ←	GTP‑binding protein YihA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,050,689	0	C	T	100.0%	17.4 / NA	8	G26E (GGA→GAA)	yihA	GTP‑binding protein YihA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

GCACGCTGCCATTTGCGCTTCATCTCTTCCGGGACTTCCGCATAACCGTACCCAGGCAAGTCAACCAGACGCTTGCCGTCAGCCACTTCAAACAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCGGATTTTTCGGCGTGAGCGTTGTAAGTAAAAGCCATACGCTTTGTGAGACATTGCCGATAGTCTTTATGCGAAATAGCAGAGAAAATTCTGCGATGC  >  NC_000913/4050473‑4050922                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          gcACGCTGCCATTTGCGCTTCATCTCTTCCGGGACTGCCGCATAACCGTACCCAGGCAAGTCAACCAGACGCTTGCCGTCAGCCACTTCAAACAGGTTGATAAGCGGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGGTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTTCGGTATCGGAAGGTAGGTGGCGAATATCAGgc                                                                                                                                                                                                           <  1:142718/249‑1 (MQ=255)                  cTTCATCTCTTCCGGGACTTCCGCATAACCGTACCCAGGCACGTCAACCAGACGCTTGCCGTCAGCCACTTCAAACAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCCGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCAACTTCAATTTCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCg                                                                                                                                                                                        >  1:71357/1‑251 (MQ=255)                                                                            ccGTCAGCCACTTCAAACAGGGTGATAAGCTGGGTGCGCCCTGGGGTTTTTGCGGCAAGAGCCAGGCTTTTCTGGTTAGTCAGCGAGCTCAGCGCGCTGGATTTACATGCGTTGGAACGGCCTGCAAAAGACACTTCAATTTCGGTATCGGAAGGTAGGTGGCGAATATCAGgc                                                                                                                                                                                                           >  1:153048/1‑174 (MQ=255)                                                                              gTCAGCCACTTCAAACAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTTCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCg                                                                                                                             >  1:77186/1‑250 (MQ=255)                                                                                                                cgcCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTTCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCGGAtttttn                                                                                          <  1:32868/251‑2 (MQ=255)                                                                                                                              tGAGGTACGAGCCAGGCTTGTCGTGTGAGGCAGCGTGTTCAGCGCGCTTGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTTCGGTATCGGAATGTATGTGGCGAATATCAGGCTCACTCATCACAAAATGCGTCTGTGGATAAGTCAAATTCGTCAAAGCTGTCTTCGCCGTCAGTAAAAGCTGTGTTGCGATTATACCGGACCCATAATAAAAGGCGGATTTTTCTGCGTGAGCGTTg                                                                             <  1:123337/250‑1 (MQ=255)                                                                                                                                                                                                         aaaaGCCACTTCAATTTCGGTATCGGAAGGTAGGTGGCGAATATCAGGAGCAGACATCAAAAAATGCGTATGTTGATCATTAAAATTCGTCAAAGCGGTCGTCTAAGTGAGTCAAAGCTTTGAGGCGATTATACCTGAACCATACTAAACGGCGGATTTTTCGGCGTGAGAGTTGAAAAGAAAAGCCATACGCTTTGTGAGACATTGCAGATAGTCTTTATGAGAAATCGCAAAGAAAAATCTTCGATGc  >  1:106640/1‑250 (MQ=255)                                                                                                                                                                                                                aCTTCAATTTCGGTATCGGAAGGAAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCGGATTTTTCGGCGTGAGCGTTGTAAGTAAAAGCCATACGCTTTGTGAGACATTGCCGATAGTCTTTATGCGAAATAGCAGAGAAAATTc          >  1:137733/1‑235 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          GCACGCTGCCATTTGCGCTTCATCTCTTCCGGGACTTCCGCATAACCGTACCCAGGCAAGTCAACCAGACGCTTGCCGTCAGCCACTTCAAACAGGTTGATAAGCTGGGTGCGCCCTGGGGTTTTTGAGGTACGAGCCAGGCTTTTCTGGTTAGTCAGCGTGTTCAGCGCGCTGGATTTACCTGCGTTGGAACGGCCTGCAAAAGCCACTTCAATTCCGGTATCGGAAGGTAGGTGGCGAATATCAGGCGCACTCATCACAAAATGCGTCTGTTGATAATTCAAATTAGTCAAAGCGGTCGTCTCCGTCAGTCAAAGCTTTGTGGCGATTATACCTGAACCATAATAAAAGGCGGATTTTTCGGCGTGAGCGTTGTAAGTAAAAGCCATACGCTTTGTGAGACATTGCCGATAGTCTTTATGCGAAATAGCAGAGAAAATTCTGCGATGC  >  NC_000913/4050473‑4050922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑