Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,971,449	G→A	G64S (GGC→AGC)	wecC →	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,971,449	0	G	A	100.0%	29.6 / NA	12	G64S (GGC→AGC)	wecC	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

CAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGC  >  NC_000913/3971210‑3971689                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 cAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCTAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTa                                                                                                                                                                                                                                       <  1:75650/251‑1 (MQ=255)                                                   aTGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATCTCGTCGAACCTGCTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                                                                                                                                     >  1:53920/1‑251 (MQ=255)                                                                 cATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                                                                                                                                       <  1:24515/251‑1 (MQ=255)                                                                                                cTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                                                                                                                                         >  1:36019/1‑250 (MQ=255)                                                                                                      aCGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAATGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                                                                                                                                  <  1:148906/251‑1 (MQ=255)                                                                                                                                                gtCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAgg                                                                                                 >  1:110800/1‑242 (MQ=255)                                                                                                                                                           ncaacATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAAt                                                                             >  1:40652/2‑251 (MQ=255)                                                                                                                                                                    ggTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAGGCGCGCTGGAGATCCTTGACTCCACCTCGc                                                                    >  1:83321/1‑251 (MQ=255)                                                                                                                                                                                                                     gCGAGTGTAGTAAAAACTGCCGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTcc                   <  1:137518/251‑1 (MQ=255)                                                                                                                                                                                                                              gtaAAAACTGCTGAAGAAAGCGGTTTTTTACGCGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGTTTGCTGTACCCACGCCGTTTAAGGTTGATCATGAGCCAGATATGACCTATGTTGAAGCGGCTGCTCCCTTCAGTGCGCCAGTGCCGAAAAAATGCGCGCTTGTGATCCTTGAATCCCCCTCTCCGGGGGGGTCAACCGAGAAGATGGCAGAACGGTTAGCAGAGATTCGTCCGGATCTCAc          <  1:27777/251‑1 (MQ=255)                                                                                                                                                                                                                              ataAAAACTGCCGTAGAAAGCGGTTTTTCACGAGCGAGCTCGAAGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGGCGGTTAAGGGCGATCATGTGCCAGATATGACCTACGTTGAATCGGCTGCGCGCTCCATTTCGCCAGTGCTGAAAAAAGGCGCGCTGGTTATCCTTGAATCCAGCTCGGCGGTGGGTGCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGTATCTCan          <  1:32136/250‑2 (MQ=255)                                                                                                                                                                                                                                        ncGTAGAAAGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAATCCACCTCACCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCgc  >  1:48907/2‑249 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 CAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGC  >  NC_000913/3971210‑3971689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑