Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	29.9 / NA	13	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/6);  total (7/6)

GGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGG  >  NC_000913/1337165‑1337594                                                                                                                                                                                                                                      |                                                                                                                                                                                                         ngCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggtgcgg                                                                                                                                                                                     >  1:42455/2‑251 (MQ=255)                                     gggCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAACAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTCACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcc                                                                                                                                                 >  1:22955/1‑251 (MQ=255)                                                 ncAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGACAGTCGTTTCTGATTATCTGACAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAACAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGCAGGCCGTATCCATCCGCTGGtt                                                                                                                                      >  1:29242/2‑250 (MQ=255)                                                                      ncTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTAACCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgc                                                                                                                >  1:102043/2‑251 (MQ=255)                                                                                                            cAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATGGGTAACTCGGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTATCTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATg                                                                          <  1:75769/251‑1 (MQ=255)                                                                                                            cAAAAGCGAAAATGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATGTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatn                                                                                                                                                  <  1:100504/179‑2 (MQ=255)                                                                                                            aaaaaGCGCAACTTACACCGTATCTCGACGAACTGTGGTTTAACCTTGTGGGATCCGGTTGTACCACCTGTATTGGTCACTCTGGGCCGCTGCCCGATCCTATCGCAACGGCAATCAAAAAAGGCGATTTAACNGTcnnnnngg                                                                                                                                                                                     >  2:100504/2‑144 (MQ=255)                                                                                                                              cGTATCTCGACGAACTGGTGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGTCTGGCCTCGCCGCCGCTGGTGGTTGCCTATg                                                                                           <  1:47550/216‑1 (MQ=255)                                                                                                                                                              ggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGc                                                                                                                                                                          <  1:56502/105‑1 (MQ=255)                                                                                                                                                                   cGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGACCGCTCCTATCGAACAGGAAATCAAAAAAGGCGATTTAAACGTCGGTGCGGTGCTGTCCGGCAACCGTCACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCa                                          >  1:156258/1‑228 (MQ=255)                                                                                                                                                                                     ttggcaCTCTGGTCCCCTGCCCGGTCCTATCGAAACGGCAATCAAAAAAGTCGATTTAACCGTCGGTTCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTAGCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCAGCCGCTGGTTGTTGCCTATGCGCTGGCGGGAAATAGGAATATCAACCTGGCTTCCGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTgg  <  1:122643/245‑1 (MQ=255)                                                                                                                                                                                             ntGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTa                                                 >  1:100242/2‑195 (MQ=255)                                                                                                                                                                                                               ctaacGAAACGGCAAGCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTAGACATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTAGTTGCCTATGCGCTAGCAGTAAATATGAATATCAGCCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGcn                            <  1:32073/194‑2 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                         GGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGG  >  NC_000913/1337165‑1337594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑