Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,905	C→T	P130S (CCT→TCT)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,905	0	C	T	100.0%	10.7 / NA	6	P130S (CCT→TCT)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/2);  total (4/2)

CGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGGACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAGCGCTGTATCTCAATCCGGTGTTTAAAG  >  NC_000913/422677‑423130                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  cgcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTCATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCTCTGATCGTTTTGCGCGTAGTCt                                                                                                                                                                                                             <  1:13465/251‑1 (MQ=255)          ccGCAGCCTGGAGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACAACGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCTCTGATCGTTTTGCGCGTAGTCTTCCTCGTg                                                                                                                                                                                                     >  1:59121/1‑250 (MQ=255)                                                                           ttACAGTTTCAAACTGCTTTGGCACGATCGCCAGAGTTGGTTTACACCGCCGGGCTTCAACCGAATGCCGCCGGCACGACTCGAGCAGTTTGCCGTCGATGCACCGGATATCGGCCCACAATCGGCTGCGGATCAGATTTTTTATAAGATCTTCTATGCTCGTTTTACGCGTAGTATTACTAGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATACTCTTGAGTGACTggg                                                                                                                                   >  1:146264/1‑251 (MQ=255)                                                                                   tCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTAAGCCGAATGCCGCCGGCAAGACTGGAGCAGTTTGACGTCGATGCACCGGATATCAGCCCACCATGGGCTGCGGATCAGATTTTTTATCAGATCTTCTCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGCTCATCTTGCGTGACTGGGATGAACCg                                                                                                                           >  1:170948/1‑251 (MQ=255)                                                                                                      tCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCAAGGATGGACCAATTTGCCCTCGATGTACCGGATATTGGCCCACACTAGGCTGCGGATCAGCTTTTTTATCTAATCTTCTCTGATCGTTTTGCGCGTAGACTTCCTCGTGaacatgaccacgatcctgt                                                                                                                                                                                  >  1:92364/1‑160 (MQ=255)                                                                                                                                                                                                            ggATCAGATTTTTTATCAGATCTTCTCTGATCGTTTGGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGGGCGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAGCGCTGTGTCTCAATCCGGTGTTTAAAg  <  1:60137/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                  CGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTTGGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGGCACGACTGGAGCAGTTTGCCGTCGATGTACCGGATATCGGCCCACAATGGGCTGCGGATCAGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTCTATTACCATCATGCAGCCGGACAAGAGATCATCTTGCGTGACTGGGATGAACCGGTCACGGCGCAGGCGGGCGGATCAACGTTCTATGGCGGCGATCTGGACGGGATAAGCGAAAAACTGCCGTATCTGAAAAAGCTTGGCGTGACAGCGCTGTATCTCAATCCGGTGTTTAAAG  >  NC_000913/422677‑423130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑