Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,908	C→T	I503I (ATC→ATT)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,908	0	C	T	100.0%	16.8 / NA	7	I503I (ATC→ATT)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

TACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGAC  >  NC_000913/3823690‑3824139                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        tACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAACTTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATTCCGCAGGAAAATATTAAGCGCGAGCTGGATCg                                                                                                                                                                                                         >  1:86489/1‑251 (MQ=255)                                                          ggCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGATCGAAAGCGAGCGCCAAACTTCGTCAGTTGCTGAAAAAACTCCAGCGTGATGATTCTGTAAGCCTGGGCAGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTATGCTGAATGAAATTCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGAc                                                                                                                                                                                   >  1:168709/1‑216 (MQ=255)                                                                 gCCCGCATGACGCTTGGCTGAACTTTGTCGTTAGCTAGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGCTTCTGTAAGCCTGGGCCGTCGTCTGCTCACCCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATTCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCgtg                                                                                                                                         >  1:136512/1‑251 (MQ=255)                                                                                    gAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATGCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATTCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACGTTTTAACGCAATGAGCGTGGTGTTCGCGAAAAATCTGc                                                                                                                      <  1:134959/251‑1 (MQ=255)                                                                                                               cgcCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATTCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATTATCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCgg                                                                                           <  1:24702/251‑1 (MQ=255)                                                                                                                             aGTTGCTGAAAAACCTCAAGCGTTATGATTCTGTAAGCCGGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATTCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCTCCGGCAGCCCAAAGCCNc                                                                             <  1:131423/251‑1 (MQ=255)                                                                                                                                                                                                        nccGTAAGCTGAATGAAATTCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGAc  >  1:48230/2‑251 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        TACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGAC  >  NC_000913/3823690‑3824139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑