Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	242,070	C→T	G412R (GGG→AGG)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	242,070	0	C	T	100.0%	30.0 / NA	12	G412R (GGG→AGG)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

CAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACG  >  NC_000913/241827‑242308                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               aaaTCGGTGCCCCCTGTTAAGCCCGGGTGAGGAAGTTGCTTTGCCCGAGCATAAGGCCTTTACCGCCGGTAATATCCATCTCATCAATAATCGACCGCTGCCCGCGGTGGGTACAGGGATACTTAACGATAGCCGACAGCACGGCAGTTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCATc                                                                                                                                                                                                                                         <  1:125398/250‑1 (MQ=255)                                    ttGCTTTGCACGAGCATAATGCCTTTACCGACGGTCATATCCATCGCATCAATAATGGACAGCTACCCGCGGTGGGTACAGTGATACTTCACGATAGCCGACAGCACGGCAGGGTTTTAGCCGAGCATAATACCGTAGGGAATCAGCGCTGCCGCAGCATCCATCACGGCGGCATTACCGGCAATACGCGCCAGAGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACTGCGAAtgtg                                                                                                                                                                                                      >  1:94081/1‑251 (MQ=255)                                                                   cgGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGAt                                                                                                                                                                       >  1:157103/1‑251 (MQ=255)                                                                                                    gcCCGCGGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGGTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGACGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTg                                                                                                                                      >  1:12382/1‑251 (MQ=255)                                                                                                         cgGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCa                                                                                                                                                                                                                                           >  1:8818/1‑145 (MQ=255)                                                                                                               gTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGcc                                                                                                                           >  1:8489/1‑251 (MQ=255)                                                                                                                                          atcACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATAAGCGATGCCGCAGCATCCACCACGCAAGCATTACCGGCCATACGCGCCAGCGGCTCTTCCCTCCTTTCCATCTTCCCCATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGAGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGCGAGGCACTccaccc                                                                                                       >  1:161969/3‑243 (MQ=255)                                                                                                                                                       tttCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGccc                                                                                    <  1:58536/250‑1 (MQ=255)                                                                                                                                                       tnnCGCCGANNATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCa                                                                                                                                                                                                                                           <  2:8818/99‑1 (MQ=255)                                                                                                                                                        ttCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGATGCACTCCACCAGCATCCGCCAGCCTTGCCCg                                                                                   <  1:151523/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccAGCGGCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGAGGCCGCGGCCTACCGAGAGGCACGCCACCAGCATCCGCCAGCCTTGCCCGGCCATTGTCGGCCCGCCGAGGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAAc        <  1:109807/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCTCTTCAATCCTTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGCGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCAATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACg  >  1:63488/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               CAATCGGTGCGCCCTGGTAAGCACGCGCCAGGAAGTTGCTTTGCCCGAGCATAATGCCTTTACCGCCGGTAATATCCATCGCATCAATAATCGACTGCTGCCCGCGGTGGGTACAGTGATACTTAACGATAGCCGACAGCACGGCAGGTTTTTCGCCGAGCATAATGCCGTAGGTAATCAGCGATGCCGCAGCATCCATCACGTAGGCATTACCGGCAATACGCGCCAGCGGCTCTTCAATCCCTTCCATCTTACCAATAGAGATTTTGAACTGACGGCGAATGTGAGCATACGCGCCGGTTGCCAGCGCTACCGATTTCACGCCGCCGGTTGAGTTGGAAGGCAGGGTGATGCCGCGGCCTACCGAGAGGCACTCCACCAGCATCCGCCAGCCTTGCCCGGCCATTTTCGGCCCGCCGATGATGTAATCGATCGGCACGAAGACATCTTTACCGCGCGTCGGTCCGTTCTGGAACGGTACG  >  NC_000913/241827‑242308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑