Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,035,891	C→T	F19F (TTC→TTT)	hchA →	protein/nucleic acid deglycase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,035,891	0	C	T	100.0%	21.3 / NA	9	F19F (TTC→TTT)	hchA	protein/nucleic acid deglycase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/3);  total (6/3)

GCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTCTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAACCTTATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCG  >  NC_000913/2035693‑2036111                                                                                                                                                                                                       |                                                                                                                                                                                                                             gcagcaTCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGAATCCATTTTCCAGCCTGGTTACAAATAGCGAACAGCTCAGTCGCAAATATAGAGACTACCCTAACTAAGCAACAATAAGGCATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTg                                                                                                                                                                          >  1:125112/1‑251 (MQ=255)  cagcaTCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAACAATAGCGACCAGCTCAGTAGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTg                                                                                                                                                                          >  1:20754/1‑250 (MQ=255)                                       gCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCTAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTg                                                                                                                                                                                >  1:23895/1‑207 (MQ=255)                                       gCTTTCGATATATTAACTCAATTTTCCAGCATGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAACCTTATCGCGGTAAACAt                                                                                                                                    >  1:45741/1‑251 (MQ=255)                                                         cAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATGTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAACCTTATCGCGGTAAACATAAAATTCTGGTGATcgcc                                                                                                                  <  1:86638/251‑1 (MQ=255)                                                                        gTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAACCTTATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTAt                                                                                                   >  1:12342/1‑251 (MQ=255)                                                                                                                                                        cAAACNNGTNNNAANNCGCAGGTCGATATTGCTGAAGATNATGCATTTTTCCCTTCAGAATATTCGCTTNGNCNATATNNCAGNCCTGTCTCTGNTCTTGATGGCGTGGACTATCCAAAACCTTATAGCGGTAAACATAAAATNCNGGTGATCGCCGCGGACGAACGTTATTTGCCGNCCGATAACGGAAAACTGTTCTCGANCGGtnnacnt                                                         >  2:123005/1‑207 (MQ=255)                                                                                                                                                        cAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATTCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAACCTTATCGCGGTAAACATAAAATTCTGGTGATCGGCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATc                                                        <  1:123005/214‑1 (MQ=255)                                                                                                                                                                                                aTGCATTTTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAGCCTTATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTTTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTttcgaattcg  <  1:170127/228‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                             GCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACTATGACTGTTCAAACAAGTAAAAATCCGCAGGTCGATATTGCTGAAGATAATGCATTCTTCCCTTCAGAATATTCGCTTAGCCAATATACCAGTCCTGTCTCTGATCTTGATGGCGTGGACTATCCAAAACCTTATCGCGGTAAACATAAAATTCTGGTGATCGCCGCGGACGAACGTTATTTGCCGACCGATAACGGAAAACTGTTCTCGACCGGTAACCATCCGATTGAAACGTTGCTGCCGTTGTATCATCTCCATGCTGCAGGTTTCGAATTCG  >  NC_000913/2035693‑2036111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑