breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 383,203 A→G W245R (TGG→CGG)  yaiP ← putative glucosyltransferase
RA 434,848 G→A A68T (GCT→ACT)  ribE → 6,7‑dimethyl‑8‑ribityllumazine synthase
RA 442,290 G→A V248I (GTC→ATC)  thiI → tRNA uridine 4‑sulfurtransferase
RA 446,036 C→T E211K (GAA→AAA)  yajR ← putative transport protein YajR
RA 448,621 G→A H14Y (CAC→TAC)  cyoC ← cytochrome bo3 subunit 3
RA 452,197 G→A A450V (GCG→GTG)  ampG ← muropeptide:H(+) symporter
RA 454,918 G→A intergenic (+129/‑215) bolA → / → tig DNA‑binding transcriptional dual regulator BolA/trigger factor
RA 460,678 G→A Q597Q (CAG→CAA lon → Lon protease
RA 463,581 G→A S556N (AGC→AAC)  ppiD → periplasmic folding chaperone
RA 464,699 G→A A100T (GCG→ACG)  fadM → long‑chain acyl‑CoA thioesterase FadM
RA 464,872 G→A Q226* (CAG→TAG)  queC ← 7‑cyano‑7‑deazaguanine synthase
RA 469,384 G→A A172T (GCG→ACG)  mdlA → ABC transporter family protein MdlA
RA 469,435 G→A A189T (GCA→ACA)  mdlA → ABC transporter family protein MdlA
RA 474,597 G→A P189S (CCG→TCG)  tesB ← acyl‑CoA thioesterase II
RA 477,894 G→A Q242* (CAA→TAA)  pdeB ← c‑di‑GMP phosphodiesterase PdeB
RA 481,484 G→A P974S (CCG→TCG)  acrB ← multidrug efflux pump RND permease AcrB
RA 485,018 G→A T201I (ACT→ATT)  acrA ← multidrug efflux pump membrane fusion lipoprotein AcrA
RA 488,839 G→A E768E (GAG→GAA mscK → potassium dependent, small conductance mechanosensitive channel
RA 492,872 G→A G261S (GGC→AGC) 
G261S (GGC→AGC) 
dnaX →
dnaX →
DNA polymerase III subunit tau
DNA polymerase III subunit tau
RA 495,221 G→A E34E (GAG→GAA htpG → chaperone protein HtpG
RA 500,604 G→A G160G (GGG→GGA gsk → inosine/guanosine kinase
RA 501,813 G→A L476L (CTG→TTG)  ybaL ← putative transporter YbaL
RA 503,911 G→A I262I (ATC→ATT fsr ← fosmidomycin efflux pump
RA 518,281 G→A intergenic (‑2/+59) cnoX ← / ← ybbO chaperedoxin/NADP(+)‑dependent aldehyde reductase YbbO
RA 531,251 C→T intergenic (‑25/+44) selU ← / ← allS tRNA 2‑selenouridine synthase/DNA‑binding transcriptional activator AllS
RA 536,970 G→A G129R (GGG→AGG)  glxR → tartronate semialdehyde reductase 2
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 615,913 G→A S586N (AGT→AAT)  entF → apo‑serine activating enzyme
RA 617,063 G→A G969G (GGG→GGA entF → apo‑serine activating enzyme
RA 618,034 G→A R1293K (AGG→AAG)  entF → apo‑serine activating enzyme
RA 620,518 G→A A224V (GCG→GTG)  fepG ← ferric enterobactin ABC transporter membrane subunit FepG
RA 628,164 T→C C157C (TGT→TGC entB → enterobactin synthase component B
RA 628,713 G→A D55N (GAC→AAC)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 636,492 G→A T26T (ACC→ACT ybdN ← putative PAPS reductase/DUF3440 domain‑containing protein YbdN
RA 640,256 G→A V168V (GTG→GTA ahpF → alkyl hydroperoxide reductase, AhpF component
RA 646,502 G→A P27S (CCC→TCC)  citT ← citrate:succinate antiporter
RA 650,230 G→A N87N (AAC→AAT citE ← citrate lyase beta subunit
RA 661,069 G→A L104F (CTT→TTT)  ybeF ← putative DNA‑binding transcriptional regulator YbeF
RA 663,759 G→A R69C (CGC→TGC)  dacA ← D‑alanyl‑D‑alanine carboxypeptidase DacA
RA 664,542 G→A L217F (CTT→TTT)  rlpA ← rare lipoprotein RlpA
RA 664,907 G→A T95M (ACG→ATG)  rlpA ← rare lipoprotein RlpA
RA 670,395 G→A L60F (CTT→TTT)  nadD ← nicotinate‑nucleotide adenylyltransferase
RA 675,064 G→A S16N (AGC→AAC)  ybeL → DUF1451 domain‑containing protein YbeL
RA 677,674 G→A R87H (CGT→CAT)  djlB → J domain‑containing protein DjlB
RA 690,238 G→A T215I (ACC→ATC)  lnt ← apolipoprotein N‑acyltransferase
RA 694,176 G→A S260S (AGC→AGT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 702,720 G→A T11I (ACC→ATC)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 703,258 G→A C118C (TGC→TGT nagB ← glucosamine‑6‑phosphate deaminase
RA 705,375 G→A A478T (GCA→ACA)  nagE → N‑acetylglucosamine‑specific PTS enzyme IIABC component
RA 705,414 G→A A491T (GCT→ACT)  nagE → N‑acetylglucosamine‑specific PTS enzyme IIABC component
RA 711,853 G→A H16Y (CAC→TAC)  ybfE ← ribbon‑helix‑helix domain‑containing protein YbfE
RA 715,516 G→A W35* (TGG→TGA ybfP → lipoprotein YbfP
RA 740,865 G→A E453E (GAG→GAA phr → deoxyribodipyrimidine photolyase
RA 769,601 G→A V542I (GTA→ATA)  mngB → alpha‑mannosidase
RA 770,032 G→A G685G (GGG→GGA mngB → alpha‑mannosidase
RA 775,011 G→A G87D (GGC→GAC)  ybgC → esterase/thioesterase
RA 776,164 G→A V106I (GTC→ATC)  tolR → Tol‑Pal system protein TolR
RA 776,311 G→A intergenic (+34/‑31) tolR → / → tolA Tol‑Pal system protein TolR/Tol‑Pal system protein TolA
RA 808,428 G→A T277I (ACC→ATC)  bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 808,808 G→A G150G (GGC→GGT bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 811,134 G→A A252T (GCG→ACG)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 817,484 G→A G147G (GGG→GGA moaA → GTP 3',8'‑cyclase
RA 821,009 G→A D73N (GAT→AAT)  ybhM → Bax1‑I family protein YbhM
RA 826,038 G→A A24V (GCG→GTG)  ybhR ← ABC exporter membrane subunit YbhR
RA 832,937 G→A A5V (GCA→GTA)  ybiA ← N‑glycosidase YbiA
RA 833,336 G→A Q89Q (CAG→CAA dinG → ATP‑dependent DNA helicase DinG
RA 834,278 G→A W403* (TGG→TGA dinG → ATP‑dependent DNA helicase DinG
RA 854,523 G→A V293M (GTG→ATG)  ybiR → putative transporter YbiR
RA 856,923 G→A E321K (GAA→AAA)  ybiT → putative ATP‑binding protein YbiT
RA 857,458 G→A S499N (AGC→AAC)  ybiT → putative ATP‑binding protein YbiT
RA 858,892 C→T G57D (GGC→GAC)  ybiU ← DUF1479 domain‑containing protein YbiU
RA 860,260 G→A R783C (CGT→TGT)  ybiW ← putative pyruvate formate lyase
RA 865,295 G→A A357V (GCC→GTC)  moeA ← molybdopterin molybdotransferase
RA 871,607 G→A S214N (AGC→AAC)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 884,298 G→A G209D (GGT→GAT)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 895,123 G→A G45S (GGT→AGT)  potG → putrescine ABC transporter ATP binding subunit
RA 896,177 G→A G15E (GGA→GAA)  potH → putrescine ABC transporter membrane subunit PotH
RA 910,295 C→T G252E (GGG→GAG)  poxB ← pyruvate oxidase
RA 911,117 G→A intergenic (‑68/+65) poxB ← / ← hcr pyruvate oxidase/NADH oxidoreductase
RA 918,891 G→A S77F (TCC→TTC)  ybjX ← DUF535 domain‑containing protein YbjX
RA 924,859 T→C R532R (CGT→CGC clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 930,390 G→A N191N (AAC→AAT cydD ← glutathione/L‑cysteine ABC exporter subunit CydD
RA 935,214 G→A G664D (GGC→GAC)  ftsK → cell division DNA translocase FtsK
RA 946,875 G→A L335L (CTG→CTA ycaD → putative transporter YcaD
RA 957,294 G→A S178N (AGT→AAT)  ycaP → DUF421 domain‑containing protein YcaP
RA 969,254 G→A E297K (GAA→AAA)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 975,438 G→A V374M (GTG→ATG)  mukF → chromosome partitioning protein MukF
RA 976,458 G→A A45T (GCG→ACG)  mukB → chromosome partitioning protein MukB
RA 981,576 G→A S177N (AGC→AAC)  ldtD → L,D‑transpeptidase LdtD
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 988,841 G→A P49S (CCT→TCT)  asnS ← asparagine‑‑tRNA ligase
RA 996,646 T→C Y100C (TAC→TGC)  ssuA ← aliphatic sulfonate ABC transporter periplasmic binding protein
RA 1,001,272 G→A G686D (GGC→GAC)  elfC → putative fimbrial usher protein ElfC
RA 1,001,463 G→A A750T (GCG→ACG)  elfC → putative fimbrial usher protein ElfC
RA 1,013,246 G→A E416K (GAG→AAG)  pqiA → intermembrane transport protein PqiA
RA 1,015,156 G→A L87L (TTG→TTA pqiC → intermembrane transport lipoprotein PqiC
RA 1,016,983 G→A A439A (GCC→GCT ycbZ ← putative ATP‑dependent protease YcbZ
RA 1,019,827 T→C Y76C (TAC→TGC)  ompA ← outer membrane protein A
RA 1,026,673 G→A P115S (CCG→TCG)  mgsA ← methylglyoxal synthase
RA 1,036,834 G→A C29Y (TGT→TAT)  hyaF → protein HyaF
RA 1,054,126 G→A L685L (CTG→TTG)  torS ← sensor histidine kinase TorS
RA 1,056,263 G→A M1M (ATG→ATA) † torT → periplasmic trimethylamine‑N‑oxide binding protein
RA 1,069,858 G→A intergenic (‑19/+2) rutG ← / ← rutF pyrimidine:H(+) symporter/FMN reductase RutF
RA 1,079,999 G→A G232D (GGT→GAT)  putP → proline:Na(+) symporter
RA 1,085,802 G→A V271I (GTC→ATC)  phoH → ATP‑binding protein PhoH
RA 1,095,879 G→A D13N (GAT→AAT)  ycdU → uncharacterized protein YcdU
RA 1,099,700 G→A D21N (GAT→AAT)  ycdY → chaperone protein YcdY
RA 1,105,328 G→A intergenic (+35/‑86) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,111,994 G→A A378T (GCG→ACG)  opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,119,585 G→A G334G (GGC→GGT solA ← N‑methyl‑L‑tryptophan oxidase
RA 1,120,661 G→A intergenic (‑75/+40) solA ← / ← bssS N‑methyl‑L‑tryptophan oxidase/regulator of biofilm formation
RA 1,122,452 G→A T52T (ACC→ACT pyrC ← dihydroorotase
RA 1,124,889 G→A A146A (GCC→GCT mdtH ← multidrug efflux pump MdtH
RA 1,126,492 G→A Q112Q (CAG→CAA yceH → DUF480 domain‑containing protein YceH
RA 1,151,298 G→A G210D (GGC→GAC)  fabG → 3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
RA 1,169,127 G→A A19T (GCC→ACC)  bhsA → DUF1471 domain‑containing multiple stress resistance outer membrane protein BhsA
RA 1,175,952 G→A G176R (GGG→AGG)  lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,193,350 G→A L142L (CTG→TTG)  mnmA ← tRNA‑specific 2‑thiouridylase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,212,851 G→A S86F (TCT→TTT)  ycgX ← DUF1398 domain‑containing protein YcgX
RA 1,214,646 G→A T277I (ACT→ATT)  bluF ← blue light‑ and temperature‑regulated antirepressor BluF
RA 1,223,271 G→A R3K (AGA→AAA)  ymgJ → uncharacterized protein YmgJ
RA 1,253,655 G→A A766A (GCC→GCT ycgV ← putative autotransporter adhesin YcgV
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
MC JC 1,299,499 Δ1,199 bp insH21 insH21
MC JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,310,045 G→A V75V (GTC→GTT kch ← K(+) channel Kch
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,353,758 T→A D254V (GAT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,401,887 C→T E457K (GAG→AAG)  abgB ← p‑aminobenzoyl‑glutamate hydrolase subunit B
RA 1,403,108 C→T A50T (GCA→ACA)  abgB ← p‑aminobenzoyl‑glutamate hydrolase subunit B
RA 1,404,682 C→T intergenic (‑117/‑59) abgA ← / → abgR p‑aminobenzoyl‑glutamate hydrolase subunit A/putative LysR‑type DNA‑binding transcriptional regulator AbgR
RA 1,410,605 C→T N365N (AAC→AAT dbpA → ATP‑dependent RNA helicase DbpA
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,508,106 G→A G442G (GGG→GGA rlhA → 23S rRNA 5‑hydroxycytidine C2501 synthase
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,538,387 C→T R156H (CGC→CAC)  narY ← nitrate reductase Z subunit beta
RA 1,538,412 C→T G148S (GGC→AGC)  narY ← nitrate reductase Z subunit beta
RA 1,539,710 C→T E961K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,697,484 C→T G186D (GGC→GAC)  hdhA ← 7‑alpha‑hydroxysteroid dehydrogenase
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,766,994 G→A I564I (ATC→ATT ydiJ ← putative FAD‑linked oxidoreductase YdiJ
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,792,977 G→A N194N (AAC→AAT btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,795,752 G→A G731G (GGC→GGT pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,834,806 G→A intergenic (+13/‑2) ydjY → / → ydjZ 4Fe‑4S ferredoxin‑type domain‑containing protein YdjY/DedA family protein YdjZ
RA 1,837,658 G→A W149* (TGG→TGA ynjC → putative ABC transporter membrane subunit YnjC
RA 1,846,553 G→A D136D (GAC→GAT topB ← DNA topoisomerase III
RA 1,962,881 A→G L31P (CTA→CCA)  flhE ← flagellar protein FlhE
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,038,955 C→T intergenic (‑132/‑1) hprR ← / → hiuH DNA‑binding transcriptional dual regulator HprR/hydroxyisourate hydrolase/transthyretin‑related protein
RA 2,112,861 C→T R67R (CGG→CGA rfbB ← dTDP‑glucose 4,6‑dehydratase 1
RA 2,129,008 C→T V215I (GTC→ATC)  wcaE ← GDP‑Fuc:2/3‑O‑Ac‑alpha‑L‑Fuc‑(1‑>3)‑alpha‑D‑Glc‑ PP‑Und alpha‑(1,4)‑fucosyltransferase
RA 2,146,847 C→T D232N (GAT→AAT)  alkA ← DNA‑3‑methyladenine glycosylase 2
RA 2,152,816 C→T D105N (GAT→AAT)  yegL ← IPR002035 domain‑containing protein YegL
RA 2,154,726 C→T V237V (GTC→GTT mdtA → multidrug efflux pump membrane fusion protein MdtA
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,181,660 C→T L189L (CTG→TTG)  yegV → putative sugar kinase YegV
RA 2,187,903 C→T R171K (AGA→AAA)  yehA ← putative fimbrial adhesin YehA
RA 2,188,502 C→T K803K (AAG→AAA yehB ← putative fimbrial usher protein YehB
RA 2,194,106 C→T L21L (CTG→CTA mrp ← P‑loop NTPase family protein Mrp
RA 2,204,319 T→C intergenic (+30/‑277) yehK → / → yehL uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,251,527 C→T R230C (CGC→TGC)  nfo → endonuclease IV
RA 2,258,643 G→A N218N (AAC→AAT psuK ← putative pseudouridine kinase
RA 2,264,035 C→T T58I (ACC→ATC)  setB → sugar exporter SetB
RA 2,267,899 C→T A24V (GCC→GTC)  yeiR → zinc‑binding GTPase YeiR
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,275,757 G→A G162E (GGG→GAG)  yejE → putative oligopeptide ABC transporter membrane subunit YejE
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,366,708 C→T R267C (CGC→TGC)  arnB → UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,509,606 C→T intergenic (‑180/‑24) glk ← / → yfeO glucokinase/putative transport protein YfeO
RA 2,509,728 C→T I33I (ATC→ATT yfeO → putative transport protein YfeO
RA 2,543,800 C→T intergenic (‑272/+32) cysP ← / ← ucpA thiosulfate/sulfate ABC transporter periplasmic binding protein CysP/oxidoreductase UcpA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,518,339 G→A L50F (CTC→TTC)  aroB ← 3‑dehydroquinate synthase
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,565,710 G→A S291F (TCC→TTC)  glgP ← glycogen phosphorylase
RA 3,582,671 G→A R270K (AGA→AAA)  yhhZ → putative endonuclease YhhZ
RA 3,586,071 G→A I251I (ATC→ATT ggt ← glutathione hydrolase proenzyme
RA 3,618,708 G→A A41T (GCG→ACG)  nikR → DNA‑binding transcriptional repressor NikR
RA 3,641,902 G→A M347I (ATG→ATA dtpB → dipeptide/tripeptide:H(+) symporter DtpB
RA 3,645,029 G→A L52F (CTC→TTC)  prlC ← oligopeptidase A
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,683,289 G→A I53I (ATC→ATT dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,731,941 G→A A271T (GCA→ACA)  xylF → xylose ABC transporter periplasmic binding protein
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,747,839 G→A G252G (GGG→GGA lyxK → L‑xylulose kinase
RA 3,812,084 G→A A159V (GCG→GTG)  yicR ← RadC‑like JAB domain‑containing protein YicR
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,347,198 T→C E43E (GAA→GAG fumB ← fumarase B
RA 4,377,545 C→T V60I (GTC→ATC)  blc ← outer membrane lipoprotein Blc
RA 4,380,268 C→T D84N (GAT→AAT)  frdB ← fumarate reductase iron‑sulfur protein
RA 4,385,242 G→A W468* (TGG→TGA yjeM → putative transporter YjeM
RA 4,394,248 C→T R61R (CGC→CGT nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,414,650 C→T Q126* (CAG→TAG)  aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,415,410 C→T P379L (CCT→CTT)  aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,434,410 A→C S100R (AGC→CGC)  ytfH → putative transcriptional regulator YtfH
JC JC 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,623,523 G→A Q199* (CAG→TAG)  lplA ← lipoate‑‑protein ligase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196358 1211520 15163 5 [4] [4] 5 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 29 [0] [0] 27 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423882–3424415 3424415 1–534 5 [4] [4] 5 rrlD 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)47 (1.060) 42/486 0.2 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)53 (1.210) 52/482 0.1 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 56677450 (1.130)14 (0.320) 11/482 2.0 21.9% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 50 (1.140)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 = 1397238NA (NA)29 (0.650) 26/486 0.7 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)27 (0.610) 26/486 0.7 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL